The Effects and Mechanism of Ba Zheng San Prescription on the Urinary Tract Infections

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Project title/計畫英文名

Project Number/計畫編號

NSC100-2320-B002-080

Translated Name/計畫中文名

八正散方劑治療泌尿道感染的效用及機轉之探討

Project Principal Investigator/計畫主持人

TE-I WENG

Funding Organization

National Science and Technology Council

Co-Investigator(s)/共同執行人

劉興華

Website

link

Start date/計畫起

01-08-2011

Expected Completion/計畫迄

12-07-2012

摘要：泌尿道感染是常見感染，常見敗血症原因，腎臟功能受損原因之一，尤其近年因不當使用抗生素增加許多抗藥性病例。若使用溫和方法治療感染，保護腎臟免於纖維化風險，改善不適症狀，對增進患者生活福祉很重要。中藥有千年歷史和溫和調理效用，近年來受到國際重視。八正散用於治療泌尿道感染出自「太平惠民和劑局方」，內有「車前子、瞿麥、萹蓄、滑石、山槴子仁、甘草、木通、大黃」。其中成分，有抑菌、免疫調節、抗氧化、抗發炎、抑制腫瘤和血管舒張作用。但關於泌尿系統保護作用研究較少。本研究有四部分：(1)八正散濃縮製劑和傳統水煎劑是否有同樣的療效。濃縮劑常添加賦形劑，在體內作用未必等於水煎劑。(2)去除”木通”後藥方效用。藥方中木通為川木通，非馬兜鈴酸科關木通，為避免民眾誤用，將研究去除木通後，該混合物是否仍有保護效用。(3)單方萃取物功效。(4)單方所含物質，例如藏紅花酸，大黃酸，甘草酸之藥理機轉。針對感染嚴重度改善，全身和泌尿系統免疫反應和過氧化壓力，預防腎臟結痂，改善膀胱感染不適症狀等方面研究。本計畫完成時，對於八正散改善泌尿道感染時不適症狀，改善腎盂腎炎發炎反應，防止腎臟結痂效用和機轉一定有所了解，對此中藥效用推廣，定有助益。
Abstract: Urinary tract infections (UTIs) are among the most common infectious diseasesacquired women. UTI is the most co‐diagnosis with sepsis in the emergencydepartment(ED). It could result in septicemia,septic shock， or the occurrence oflong term complications such as chronic renal failure. These complications are mainlyproduced following the formation of renal scarring in patients with UTIs. Patientssuffered from UTIs complain of frequency and urine retention. These symptomsimpair their quality of life. Uropathogens are increasingly becoming resistant tocurrently available antibiotics is a serious problem. Ba Zheng Son(BZS), an importanttraditional Chinese medicine formula for the treatment of UTIs, contains Chineseplantain, large pink, talc, rhudarb, cape jasmine fruit, herba polygoni avicularis, caulisclematidis, slender licorice root, and common rush. The components of BZS arepotent in anti‐diabetes, anti‐oxidants, anti‐inflammation, antiviral agents,antibacterial agents and vasorelaxants. However, to date, there have been fewstudies on the mechanism of BZS on UTIs and associated complications.There are four parts in our project. First, we will investigate the biological effects oftraditional herbal decoction and the commercial extract of BZS. There are twodosage forms of BZS in Taiwan. One is traditional herbal decoction, and the other iscommercial extract. The biological effects of these two dosage forms may bedifferent. Secondly, we will determine the effects of BZS except Caulis clematidisarmandii on UTIs. Although they have similar names, Caulis clematidis armandii isdifferent plant from Caulis Aristolochiae Manshuriensis, a nephrotoxic agent. CaulisAristolochiae Manshuriensis is prohibited from the market. The patient may bemisuse while they take the prescription of BZS. We will research the biological effectof BZS except Caulis Clematidis armandii. Thirdly, we will research the individualeffects of the extracts. The final step, we will investigate the mechanism of crocetin,rhein, and glycyrrhizin extracts from cape jasmine fruit, rhudarb, and licorice rootseparately.We will evaluate whether traditional herbal decoction, the commercial extract, theindividual extracts, and the compound of the components of BZS attenuate theinflammatory severity of acute pyelonephritis in rats, and explore the possiblemechanism. To further evaluate their protective properties, we will determine theprotective effects against bacterial induced renal fibrosis signals, and the mechanismwill be also studied. The contractions of isolated rat detrusor strips evoked by fieldstimulations (EFS) or by exogenous agonist and immuno blotting for the detection ofprotein expression in the bladder and kidneyere observed in the short (1 h) and long(24 h) term. We will test the hypothesis of the possible improvement of the detrusordysfunction by BZS and discuss the possible mechanism.Taken together, we hope these experiments will support a therapeutic effects and mechanism of BZS for the treatment of human urinary tract infection associateduncomfortable symptoms and severe complication such as acute pyelonephritis,renal fibrosis and sepsis, and further understanding the anti‐inflammatory andanti‐oxidant mechanism of these drugs.

Keyword(s)

膀胱
細菌
內毒素
八正散
urinary bladder
bacteria
lipopolysaccharide
Ba Zheng Son

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The Effects and Mechanism of Ba Zheng San Prescription on the Urinary Tract Infections

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