Abstract: Urinary tract infections (UTIs) are among the most common infectious diseasesacquired women. UTI is the most co‐diagnosis with sepsis in the emergencydepartment(ED). It could result in septicemia,septic shock， or the occurrence oflong term complications such as chronic renal failure. These complications are mainlyproduced following the formation of renal scarring in patients with UTIs. Patientssuffered from UTIs complain of frequency and urine retention. These symptomsimpair their quality of life. Uropathogens are increasingly becoming resistant tocurrently available antibiotics is a serious problem. Ba Zheng Son(BZS), an importanttraditional Chinese medicine formula for the treatment of UTIs, contains Chineseplantain, large pink, talc, rhudarb, cape jasmine fruit, herba polygoni avicularis, caulisclematidis, slender licorice root, and common rush. The components of BZS arepotent in anti‐diabetes, anti‐oxidants, anti‐inflammation, antiviral agents,antibacterial agents and vasorelaxants. However, to date, there have been fewstudies on the mechanism of BZS on UTIs and associated complications.There are four parts in our project. First, we will investigate the biological effects oftraditional herbal decoction and the commercial extract of BZS. There are twodosage forms of BZS in Taiwan. One is traditional herbal decoction, and the other iscommercial extract. The biological effects of these two dosage forms may bedifferent. Secondly, we will determine the effects of BZS except Caulis clematidisarmandii on UTIs. Although they have similar names, Caulis clematidis armandii isdifferent plant from Caulis Aristolochiae Manshuriensis, a nephrotoxic agent. CaulisAristolochiae Manshuriensis is prohibited from the market. The patient may bemisuse while they take the prescription of BZS. We will research the biological effectof BZS except Caulis Clematidis armandii. Thirdly, we will research the individualeffects of the extracts. The final step, we will investigate the mechanism of crocetin,rhein, and glycyrrhizin extracts from cape jasmine fruit, rhudarb, and licorice rootseparately.We will evaluate whether traditional herbal decoction, the commercial extract, theindividual extracts, and the compound of the components of BZS attenuate theinflammatory severity of acute pyelonephritis in rats, and explore the possiblemechanism. To further evaluate their protective properties, we will determine theprotective effects against bacterial induced renal fibrosis signals, and the mechanismwill be also studied. The contractions of isolated rat detrusor strips evoked by fieldstimulations (EFS) or by exogenous agonist and immuno blotting for the detection ofprotein expression in the bladder and kidneyere observed in the short (1 h) and long(24 h) term. We will test the hypothesis of the possible improvement of the detrusordysfunction by BZS and discuss the possible mechanism.Taken together, we hope these experiments will support a therapeutic effects and mechanism of BZS for the treatment of human urinary tract infection associateduncomfortable symptoms and severe complication such as acute pyelonephritis,renal fibrosis and sepsis, and further understanding the anti‐inflammatory andanti‐oxidant mechanism of these drugs.