Abstract
摘要:現今具備高解析度(微米級)之光學醫學影像技術,主要為共軛焦顯微鏡(confocalmicroscopy)及光學同步斷層攝影(opticalcoherencetomography)。共軛焦顯微鏡技術之優點:高解析度(<=10 微米)、可將患者組織染色取得螢光影像與光譜資訊。其缺點:取得患者抹片需要大量掃描之耗時系統、系統價格高昂。光學同步斷層攝影法之優點:高解析度(約10-20 微米)、相對低成本。其缺點:無法獲得光譜訊息與螢光影像、需要大量掃描之昂貴耗時系統。「高速光學體全息三維空間生醫影像系統(Fast optical holographic three-dimensionalspatial biomedical imaging)」是新型生醫影像系統,擁有高速(high-speed)能力取得組織(tissuesamples)的多層空間訊息並同時獲得相關光譜特性,並同時具備共軛焦顯微鏡及光學同步斷層攝影法之綜合優點:(1) 不需耗時複雜掃描,透過多工全像術可取得三維影像及相關光譜訊息。(2) 高解析度(微米級)。(3) 可取得生物組織之螢光影像。(4) 相對低成本。本研究計畫主要發展針對癌症病變(cancerousortumorous)組織早期的多層偵測之新型顯微影像系統:「高速光學體全息三維空間生醫影像系統」。其研究重點:將製作與運用全像光柵(holographicgrating)作為空間與光譜濾波器。此關鍵元件可精確取得測試物之波前(wavefront)和相位(phase)訊息,並可降低不相關訊息之背景雜訊(backgroundnoise)。進而運用三維空間(3Dspatial)與高解析度光譜(hyper-spectral)解析能力,高速取得病變組織之三維影像與相關光譜訊息。「高速光學體全息三維空間生醫影像系統」是結合光學、工程、生醫等相關技術的計畫。本研究計畫預計由台灣大學醫學院的光電醫學研究中心與北京清華大學機械工程學院的精密工程學系光電工程研究所合作進行生醫、工程與光學科技的跨領域結合,期望「高速光學體全息三維空間生醫影像系統」將在學術上、產業應用與生物醫學上具有卓越的貢獻。
Abstract: Cancer is one of the leading causes of death in the world. The five-year survival rate isimproved to 95% if the cancer is discovered at an early, localized stage.Therefore, mucheffort has been expended in recent years developing imaging methods and instrumentation forearly detection.Two most primary optical imaging modalities, optical coherence tomography(OCT) and confocal, have shown great promise for detecting abnormal and tumorousstructures in tissue. Confocal offers high resolution image data, and can obtain fluorescentinformation of tissue samples. However, confocalis expensive, and it needs complicated andtime-consuming scanning. OCT can produce high-resolution (~10μm) cross-sectional imagesof organ sites. However, OCT is a coherent imaging technique, and thus cannot providerequired fluorescence images and related spectral information of an interested biologicalsample.“Fast Optical holographic spatial-spectral biomedical imaging” is a novel imagingtechnique, which offers capability to acquire multi-depth resolved images and spectralinformation of tissue samples. The system provides complimentary benefits of confocal andOCT, which are1. high-speed to extract 3Dspatial images and spectral information through holographicmultiplexing, without time consuming scanning,2. high resolution,3. easy to acquire fluorescence images, and4. simple, low-cost and robust.The key aspect of multiplexed volume holographic imaging that motivates this proposalis the ability to acquire high-resolution spatial 3D images without scanning, and providehyper-spectral information. The holographic gratings can detect wavefront and phase fromregion of interest in a tissue samples; meanwhile it will reduce background noise.The collaboration, between the Center for Optoelectronic Biomedicine in the College ofMedicine at National Taiwan University and Department of Precision Instruments in theSchool of Mechanical Engineering at Tsinghua University, is unique in its experience fordeveloping the proposed instrument. The proposed “Fast optical holographic spatial-spectralbiomedical imaging” has significant advantages over conventional optical imaging systems inthat it does not require complicated scanning or intensive computational reconstructiontechniques, and can extract 3D spatial images in one shot and hyper-spectral information inhigh-speed format.This is a revolutionary advance that we believe will open the way for betterunderstanding of the onset of cancer and biological tissue morphology as well as other clinicaluses through high-speed observation capability.
Keyword(s)
三維空間影像(3D
spatial
imaging)
高解析度光譜(hyper-spectral)
全像光柵(holographic
grating)
螢光(fluorescence)
共軛焦顯微術(confocal)
與光學同步斷
層攝影術(OCT)3D
spatial
imaging
hyper-spectral
holographic
grating
fluorescence
confocal
and
OCT