摘要:生醫用途實驗用禽畜的生產是生技醫藥產業發展的基石。我國無特定病原(specific pathogen free, SPF)或最少疾病(minimal disease, MD)的各項禽畜相關疾病界定及檢測方法尚未完整建立。因此本計畫配合其他研究群組,將協助發展生醫用途實驗用禽畜包括疾病、遺傳及營養等監控、檢測標準及驗證等制度之建立。其中為提升我國所生產之SPF雞及雞胚胎之品質,建立第一群腺病毒(group 1 Adenovirus)、第二型禽副黏液病毒、雞貧血病毒(CAV)、馬立克病病毒(MDV)四種疾病抗體檢測方法,並對行政院農業委員會家畜衛生試驗所動物用藥品檢定分所之SPF雞進行18種疾病(新城病(ND)、傳染性支氣管炎(IB)、傳染性喉頭氣管炎(ILT)、傳染性華氏囊炎(IBD)、產蛋下降症候群(EDS)、傳染性滑膜炎(MS)、禽淋巴性白血病(ALL)、里奧病毒感染症(REO)、雛雞白痢症(PD)、慢性呼吸道疾病(MS)、家禽腦脊髓炎(AE)、家禽流行性感冒(AI)、禽鼻氣管炎(ART)、網狀內皮細胞病毒(RE)感染症第一群腺病毒(group 1 Adenovirus)、第二型禽副黏液病毒、雞貧血病毒(CAV)、馬立克病病毒(MDV))抗體之檢測。遺傳監控方面收集家衛所動物用藥品檢定分所SPF兔、新化總所MD兔及彰化種畜繁殖場MD鵝的血液,抽取DNA,並設計適當的微衛星標幟引子進行PCR分析,分析遺傳的多型性,做為遺傳監控的基礎資料,並研發成遺傳監控的試劑套組的可行性。在營養監控分面,將收集蘭嶼豬各組織器官能量與蛋白質代謝相關基因的表現,以加強蘭嶼豬相關的基礎資訊建立。計劃以CRD設計,設定三個能量濃度(2500, 2700與2900 kcal/kg) 撘配二種粗蛋白濃度(14.5%與16.5%),試驗期由10週齡至20週齡為止。20週齡時,收集腦下腺、肝臟、肌肉及脂肪組織分析能量代謝相關基因表現。
另為因應我國生技醫藥產業發展之所需,協助規劃符合國內生技醫藥產業發展需求及國際品質要求之畜禽實驗動物供應體系認證制度。本年度希望達成下列目標:A. 以「實驗用畜禽生產標準化生產供應作業指南」為準則,導入TAF驗證制度,建立實驗用畜禽生產供應單位品質觀念,提升包括疾病、遺傳、營養等品質管理,完成我國實驗畜禽動物機構品質驗證制度之建立。
B.實施機構品質驗證種子師資訓練課程。
Abstract: One of the foundations for development of biomedical industry is the good and high quality supply of experimental animal. In Taiwan, the production system of specific pathogen free (SPF)] or minimal disease (MD) experimental animal is not well established, yet. Through the collaboration among several research teams, the aims of this project include disease, nutritional and genetic monitoring; definition and or establishment the standard assays; and establishment the evaluation system for production units. To enhance the quality of SPF chickens and chicken embryo production in Taiwan, we propose to establish the antibody detection method of group 1 adenovirus, type II avian paramyxovirus, chicken anemia virus (CAV), and Marek`s disease virus (MDV) during this year’s project. We will also test the SPF chickens produced by the Animal Drug Inspection Branch Institute of the National Veterinary Institute, Council of Agriculture for the antibodies against 18 avian diseases. The disease examined will include Newcastle disease (ND), infectious bronchitis (IB), infectious laryngotracheitis (ILT), infectious bursal disease (IBD), egg drop syndrome (EDS), infectious synovitis (MS), avian lymphoid leukemia (ALL), reovirus infection (REO), pullorum disease (PD), chronic respiratory diseases (MS), poultry encephalomyelitis (AE), avian influenza (AI), avian rhinotracheitis (ART), reticuloendotheliosis (RE), group 1 adenovirus, type II avian paramyxovirus, chicken anemia virus (CAV), and Marek`s disease virus (MDV). In genetic monitoring, blood will be collected from SPF rabbit, MD rabbit and MD goose. DNA will be isolated to evaluate the genetic diversity within animals with PCR by designed primers pairs of microsatellite loci. The genetic monitoring kits will be set as a result of the information of microsatellite analysis. Another object of the project attempts to elucidate the expression of metabolic-related genes (energy and protein) and set up the basic information for Lanyu pig. The project will be designed as CRD with three energy levels (2500, 2700 and 2900 kcal/kg) and two protein levels (14.5% and 16.5%). The experimental period will start at 10 weeks of age and last for 10 weeks. At the end of experiment, pituitary gland, liver, muscle and adipose tissue will be collected to analyze the energy-related gene expressions.
This plan will assist in programming centification of standardized provision system for the use of agricultural animals in biomedical research and teaching, according to requirement of biomedical industry’s development. The goal of this year: A. To complete the draft of establishment of certification of laboratory agricultural animal facilitie’s quality under the guideline of standardized provision system for the use of agricultural animals in biomedical research and teaching. Improve the quality management of disease, genetics, and nutrition in laboratory by inducting TAF certification. B. To establish the teacher training of certification of facilitie’s quality.