摘要：很多衛生害蟲是屬於世界性分布的，我們與捷克科學院昆蟲所的研究員Dr. Ivan Gelbic共同合作，針對這些廣泛性分布的衛生害蟲的生殖生理調控機制，做深入的瞭解，希望能基於對這些機制的掌控，共同開發有效的防治方法。目前先規劃捷克方面，從研究多年之家蚊（Culex pipiens）進行生殖生理的研究。而我們則針對熟悉的德國蜚蠊（Blattella germanica）來研究。德國蜚蠊為世界性分布之衛生害蟲，目前仍困擾著世界各地人類的生活。本計畫擬針對德國蜚蠊之生殖副腺與其生殖表現及活動行為日週律動之關係，進行深入的探討。一般認為昆蟲之雄性生殖副腺分泌物與精包的合成有關，而雌性副腺則可能與卵鞘的形成及抗菌蛋白之分泌等有關。因此，如能對德國蜚蠊之副腺進行深入研究，則有利於對生殖系統之掌控，而有助於防治研究工作之進展。本計畫將先對雌雄副腺之外部形態做觀察及描述，再進行蛋白質分析。並以實驗探討副腺蛋白對雌蟲本身之卵鞘形成、子代數、子代存活及內分泌（如青春激素、蛻皮激素）的影響。也將探討對雄成蟲本身之精包形成、交尾時間及子代存活，所造成之影響。雌雄副腺之活性，是否有日週律動的現象？而其與生殖週期
Abstract: Many medical importance pests are world-wide distributed. In this project, we will cooperate with Dr. Ivan Gelbic in the Institute of Entomology, Czech Academy of Sciences, to investigate on the physiological mechanisms of reproduction of the medical importance pests. We will look into those mechanisms and hope to develop efficient pest control methods based on the results. Our Czech’s counterpart will conduct a study on the reproductive physiology of mosquito Culex pipiens, the species which they have been studied for many years. In our laboratory, we will study on the German cockroach (Blattella germanica), our favorite research insect for the last 10 years. The German cockroach is a cosmopolitan household species, which is closely associated with our daily life. In this project, we investigate the function of accessory glands on reproductive performances and locomotor circadian rhythm of the German cockroach. The basic function of the accessory glands of male insects is to provide materials for the production of the spermatophore. In females, the accessory glands are important in the formation of ootheca and secretion of antibacterial proteins. Therefore, it is of great importance to study the accessory glands for understanding the mating system of the pest and consequently making contribution to further development of pest control. Firstly, we will observe and describe the morphology of the accessory glands for both sexes of the German cockroach. The relationships between accessory gland secretion and ootheca formation, egg number, survivorship of offspring and endocrine secretion (e.g. JH, ecdysone) for females, and spermatophore formation, time of copulation and survivorship of offspring for males will be examined. The daily secretion rhythm of the accessory glands will be investigated to reveal its circadian rhythm. In addition, the effects of male accessory gland secretion on female reproductive performances (e.g. re-mating rate, egg number, survivorship of offspring) and competition with other males’ sperms will be our focal point of research. In the last year of this project, we will conduct the bioassay for specific proteins of the accessory glands, including antibacterial analysis, reproductive performances and the effects on locomotor circadian rhythm of females. We will also purify the proteins and then, analyze and sequence the amino acids. Using the antibody of specific protein, we will be able to trace the localization of the specific protein during and after the process of copulation. We expect to understand more about the mating system of the German cockroach through the proposed studies. Furthermore, the effects of accessory glands from both male and female on reproductive performances and locomotor circadian rhythm will be elucidated.