(4) 高頻生物感測器的晶片大小在 6mm × 6mm之內。
Abstract: As the fast development of the molecular biology, it becomes more demanding for a rapid and accurate instrument that can used to detect biomolecules. Nevertheless, there are tremendous challenges for such a commercial instrument to face such as miniaturization of instrument and contamination of detected sample on the instrument. Therefore, a novel portable and miniature bio-detection system is proposed in this project. With highly integrations of RF MEMS technology, nanoparticles and micro fluidic devices, the proposed system is expected to be capable of overcoming those obstacles. The major principles applied to this system are described as follows. A biochip with a coplanar waveguide circuit was established on a glass substrate by using a MEMS technology. Specific hybridization among ssDNA is used to establish bio-nanoparticle-conjugate nanostructures on the detection surface of biochip. When the bio-nanoparticle-conjugate nanostructures are established on the detection surface of the coplanar waveguide circuits, the electromagnetic behaviors of the circuits are changed due to the existence of the bio-nanoparticle-conjugate nanostructures. In addition, the electromagnetic behavior of circuit depends on how dense of nanostructure can be established on the surface, which is affected by the concentration of target DNA strand. Therefore, the system can be used to quantify the concentration of target DNA in the tested sample. This project is planed to be completed within 3 years. The mission of the first year is to design and implement a RF biosensor consisting of coplanar waveguide circuits; meanwhile to define the sensitivity and reproducibility. The mission of the second year is to apply the gold nanoparticles and magnetic nanoparticles to enhance the sensitivity of the current devices. The aim of the third year is to integrate the micofluidic system to be more compact.
The major outcomes that can be achieved by this project are:
(1) The implementation of a CPW device that is operated at a UHF band.
(2) Verification of the quantification ability of our developed biochip, the detection limitation could be as low as 1fM.
(3) The resolution of the amplified sensing signals should be 100 fold of those before amplification.
(4) The chip size is within the area of 6mm × 6mm.
(5) The volume of the sample container is larger than 100μL.
(6) A integrated system with microfluidic devices has the capability for wireless transmission of detection signals of target biomolecules in the tested sample.