Abstract: According to the report from the World Health Organization (WHO), HIV, tuberculosis (TB) and malaria are the top 3 lethal diseases globally. Among them, the prevention of TB remains great challenging based on the highly infectious via saliva and the lack of effective detection platform. Even now there was an estimated 10 million people developed TB in the world, and approximately 1.4 million people died from TB in 2015.
Current TB detection methods include mainly smear microscopy, a culture of bacilli and molecular diagnostics. The smear microscopy approach is a cost-effective way to obtain the results in minutes. However, this identification has a sensitivity of only approximately 34–80%. Bacillus is the standard method for TB detection in a clinical center. However, it takes 9-16 days to obtain the culture results. Molecular diagnostics adopting polymerase chain reaction to amplify the specific MTB target DNA from extracted sputum possesses a sensitivity of 90%. However, the turnaround time, including multiple hybridization processes requires 2-5 hours, and the sophisticated infrastructure and trained personnel limits the feasibility. Due to the majority of newly infected cases is in the resource-limited environments, a rapid, simple, low cost, and highly accurate on-site detection platform for TB diagnosis is needed for better infection control.
To overcome these challenges, here I propose the microfluidic system that provides the following critical techniques including (a) sample preparation via the bifurcation fluidic manipulation together with (b) high specific DNA and protein probe selection, followed by (c) the integration of thermoplastic substrate and microfluidic paper-based analytical devices for rapid and sensitive TB diagnostics in resource-limited settings.
infectious disease diagnosis
miniaturized analytical technique