Research Project: 提昇飼料牧草品質及其檢測技術--應用產多不飽和脂肪酸單胞藻類於家禽飼料之開發
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摘要：魚油中二十碳五烯酸(EPA)、二十二碳六烯酸(DHA)可用以強化蛋黃EPA、DHA含量。惟魚油的DHA含量有限(10%)，欲達高量DHA強化，常造成蛋具魚腥味。添加藻類DHA油亦可增進蛋黃中DHA含量，惟藻類DHA油較昂貴，其實用性有待改善。本計劃希望藉由篩選可生產多不飽和脂肪酸之單胞藻類及其他微生物，並建立簡易之產多不飽和脂肪酸微生物的培養方法，以生產飼料用之生物製品，提供便宜之多不飽和脂肪酸來源，俾能提升蛋品之利用價值。
首先篩選四種可生產多不飽和脂肪酸之單胞藻類及其他微生物並進行培養。選擇一或多個可供生產最高量EPA或DHA之微生物，以改變不同培養條件進行培養以建立其最適之培養條件。將原先於三角錐瓶中培養之最適條件，於生物反應器培養測試並調整培養條件，以大量培養微生物。
將所產得之微生物製品分別添加0、1%或3%於產蛋來航雞飼料中，進行初步飼養試驗以評估這些製品於家禽飼料之應用性。飼養期間紀錄產蛋率，並於1、2及3週時分別測定蛋黃中脂肪酸的組成。餵飼三週後犧牲動物，測定肝臟中參與脂質生合成基因的表現。
本計劃將可篩選出能生產多不飽和脂肪酸之單胞藻類及真菌等微生物，建立個別微<br> Abstract: Eicosapentaenoic Acid (EPA) and docosahexaenoic acid (DHA) in fish oil can be utilized to enrich egg yolk EPA and DHA content. Fish oil contains limited amount of DHA (10 %). To enrich yolk DHA content in high amount, fish odor is a regular concern. Utilization of algal DHA oil in poultry diet can also enhance yolk DHA content. Because the cost of algal DHA oil is high, its practicability need to be improved. The current proposal is designed to examine strains of polyunsaturated fatty acid(PUFA) – rich microalgaes or other microorganisms, and establish simple cultural methods of these microorganisms to produce bio-product for poultry diet. The bio-product can supply inexpensive PUFA to raise value of egg products.
We will screen 4 strains of PUFA – rich microalgae or other microorganisms and culture them. Then choose one strain that can produce high volume of PUFA and establish the optimal cultural condition of the microorganism by changing cultural conditions. The optimal cultural conditions of the microorganism in the flasks will be applied to culture in bio-reactor. Adjust the cultural conditions in bio-reactor and mass culture the microorganism.
Levels of 0, 1, 3 % bio-product from the microorganism will be added to the diets for leghorn hens to estimate the practicability of these bio-product in poultry diets. Record the egg production and collect eggs at 1, 2, 3 week for yolk fatty acid composition analysis. At the end of the experiment, the birds will be killed and the expression of hepatic lipogenesis-related genes determined.
The current proposal will select an optimal strain of microalgae or fungus which can produce high PUFA. It will be established the optimal cultural conditions of producing PUFA of these microorganisms. Mass culture of microorganisms to produce bio-product that would support the demands of further research. Addition of these bio-products in the diet of laying hens can enrich egg yolk PUFA content. It will help to produce low cost, high DHA content functional eggs and raise the value of egg products.
Keywords
二十二碳六烯酸
藻類
來航雞
DHA
algae
Leghorn hens
藻類
來航雞
DHA
algae
Leghorn hens