建構A型流感病毒非結構蛋白(NS1)及病毒顆粒核蛋白(NP)之泛用型流感病毒感染早期雙抗原蛋白檢測套組

Project title
建構A型流感病毒非結構蛋白(NS1)及病毒顆粒核蛋白(NP)之泛用型流感病毒感染早期雙抗原蛋白檢測套組
Internal ID
111農科-1.6.1-科-aH
Principal Investigator
Start Date
January 1, 2022
End Date
December 31, 2022
Organizations
Partner Organizations
Council of Agriculture, Executive Yuan
Keywords
Avian influenza virus
Gold nanoparticles
Antigen rapid test
Monoclonal antibody
Immunochromatographic strip
Description
To develop avian influenza virus (AIV) nucleoprotein and non-structural protein NS1 detecting method or kit which will be based on highly specific and sensitive monoclonal antibody against NP and NS1. The diagnostic method is capable of applying on all susceptible animals and all AIV subtypes. Also, the method can be an early detection tool of AIV infection and play an important role of disease early warning system. To reach the goal of obtaining the chromatographic based rapid test kit, the Strip, for the field application of real-diagnosis and real-implementation of disease control measures, the second year of this two-year project aims for the work with the cooperated company on the development of NP Strip and supplemented by the development of NS1 Strip. Finally, two NP and NS1 「Strip prototype」each have been completed and examined on eight test bio-samples to obtain the sensitivity (Sn) and specificity (Sp) this year. Those bio-samples include six subtype of IAV, H1N1/H1N2/H3N1/H5N2/H6N1/H7N9 cell lysates, experimentally infected chicken swab and IBV. The Sn of both NP 「Strip prototype」are all far-higher than that of the NS1「Strip prototype」. Also, both NP 「Strip prototype」show the absolute Sp from samples of IBV and SPF. Based on the result, the cooperator should get the full access of the key bio-materials, six different monoclonal antibodies, after the cooperation period. The massive productionof the「 NP Strip」can be adjusted then, and marketed to apply on the surveillance of animal influenza in the field.

臺大位居世界頂尖大學之列，為永久珍藏及向國際展現本校豐碩的研究成果及學術能量，圖書館整合機構典藏（NTUR）與學術庫（AH）不同功能平台，成為臺大學術典藏NTU scholars。期能整合研究能量、促進交流合作、保存學術產出、推廣研究成果。

To permanently archive and promote researcher profiles and scholarly works, Library integrates the services of “NTU Repository” with “Academic Hub” to form NTU Scholars.

總館學科館員 (Main Library)
醫學圖書館學科館員 (Medical Library)
社會科學院辜振甫紀念圖書館學科館員 (Social Sciences Library)

開放取用是從使用者角度提升資訊取用性的社會運動，應用在學術研究上是透過將研究著作公開供使用者自由取閱，以促進學術傳播及因應期刊訂購費用逐年攀升。同時可加速研究發展、提升研究影響力，NTU Scholars即為本校的開放取用典藏（OA Archive）平台。（點選深入了解OA）

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