Tumour Necrosis Factor-Alpha-Induced Apoptosis in Cord Blood T Lymphocytes: Involvement of Both Tumour Necrosis Factor Receptor Types 1 and 2
Resource
BRITISH JOURNAL OF HAEMATOLOGY v.115 n.2 pp.435-441
Journal
BRITISH JOURNAL OF HAEMATOLOGY
Journal Volume
v.115
Journal Issue
n.2
Pages
435-441
Date Issued
2001
Date
2001
Author(s)
YANG, YA-CHIEN
HSU, TSUEY-YING
CHEN, JEN-YANG
YANG, CZAU-SIUNG
LIN, RONG-HWA
Abstract
Cord blood T cells are much more likely to be induced to apoptosis in vitro than adult T cells. Nevertheless, the expression of Fas is markedly lower on cord blood lymphocytes than on peripheral blood lymphocytes. In the current investigation, we determined the capacity of tumour necrosis factor-alpha (TNF-alpha) to induce apoptosis in human naive T cells in cord blood, and assessed the roles of two distinct TNF receptors (TNFRs) in mediating death signals. After activation, cord blood T cells were sensitive to TNF-alpha -induced apoptosis, and interleukin 2 (IL-2) could prevent this apoptotic response. Both TNFR1 (p55) and TNFR2 (p75) expressed on activated cord blood T cells were able to transmit apoptotic signals. Moreover, a synergistic effect was observed by a combination of TNFR1- and TNTFR2- signals. Additionally, CD4(+) T cells showed higher sensitivity to TNTR-mediated apoptosis than CD8(+) T cells. These data suggest that TNF-alpha probably is a mediator of apoptosis in cord blood T cells in vivo and may contribute to the low incidence of graft-versus- host disease in cord blood transplantation.
Subjects
cord blood
T cells, apoptosis
TNFR1
TNFR2
VERSUS-HOST DISEASE
UNRELATED DONORS
SDGs