Correlation of flow cytometric and microdensitometric DNA content analysis in lung cancer
Journal
Analytical and Quantitative Cytology and Histology
Journal Volume
16
Journal Issue
1
Pages
25-28
Date Issued
1994
Author(s)
Kuo S.-H.
Lee Y.-C.
Liu Y.-R.
Lee Y.-T.
KWEN-TAY LUH
How S.-W.
Abstract
The DNA histograms of 29 histologically verified primary lung cancers were analyzed with flow cytometry and microdensitometry. Fresh tumor tissues were imprinted on the slide and air dried for modified Feulgen staining. Then the smears were scanned with a scanning microdensitometer at a wavelength of 550 nm. The S-phase fraction value was estimated from the DNA histogram with a cumulated probability scale. The remaining parts of the tumors were minced and prepared for dispersal as a single cell suspension for propidium iodide staining. The tumor cells were then analyzed with a flow cytometer at 488 nm. The correlation between the analyses measured with these two methods showed r = .75 for the DNA index, r = .71 for the S-phase fraction and r = .61 for the G0G1 population; P < .001. The DNA indices, S-phase fraction values and G0G1 populations obtained with flow cytometry and microdensitometry correlated well.
SDGs
Other Subjects
propidium iodide; adult; aged; article; cell cycle g0 phase; cell cycle g1 phase; cell cycle s phase; clinical article; dna content; feulgen staining; flow cytometry; human; human cell; lung cancer; microdensitometry; priority journal; Adenocarcinoma; Aged; Carcinoma, Small Cell; Carcinoma, Squamous Cell; Densitometry; DNA, Neoplasm; Flow Cytometry; Human; Lung Neoplasms; Middle Age; Propidium; S Phase
Type
journal article