Antihypertensive effect of enzymatic hydrolysates from soft-shelled turtle powder
Date Issued
2009
Date
2009
Author(s)
Chang, Yu-Hsun
Abstract
Chinese soft-shelled turtle (SST) is a traditional Chinese medicine, and was also confirmed that possessed various kinds of physiological functions. The objectives of this study were to produce anti-hypertension effect of SST powder hydrolysate digested by papain. SST powder was hydrolyzed under various conditions of hydrolysis time and enzyme-to substrate ratio (E/S, %) according to a response surface methodology (RSM) 2 factors rotatable central composite design, and hydrolysate were evaluated by peptide concentration and ACE inhibitory activity. The better hydrolysate conditions of SST powder hydrolysate digested by papain are pH 5.5, 60℃, 5% E/S for 3 hours. The SST powder showed limited IC50 value of ACE inhibitory activity were 16.2±1.6 mg/mL. The SST powder hydrolysate derived from hydrolysis of papain obviously increased ACE inhibitory activity, and IC50 were 2.87±0.32 mg/mL. In short term animal experiment, single oral administration of 500 mg/kg BW of SST powder hydrolysate to spontaneously hypertension rat (SHR) was significantly lowered systolic blood pressure at 5th hours. In long term experiment, the experiment group feed diet containing 0.6% SST powder hydrolysate was noticeable decreased systolic blood pressure compared to control group at 4th and 6th weeks (p<0.05) and significant suppression at 8th weeks (p<0.01). The ACE activity of SHR feed diet containing 0.6% SST powder hydrolysate were lower than control group. The results suggested that SST powder hydrolysate digested papain can modulate blood pressure.
Subjects
Soft-shelled turtle
angiotensin I-converting enzyme
papain
hypertension
response surface methodology
bioactive peptide
Type
thesis
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