Protective Effects of Genistein Against Oxidative Damages of Salivary Gland Acinar Cells.
Date Issued
2015
Date
2015
Author(s)
Shih, Yu-Kai
Abstract
Back ground：There are so many reasons cause xerostomia like radiation therapy duo to head neck cancer or autoimmunity disease such as Sjogren’s syndrome. Radiation therapy will cause the rise of R.O.S. resulting the hypofunction of salivary gland. Other research was also noted the rise of 8-OHdG a DNA oxidation maker in saliva of the the patient with Sjogren’s syndrome. It’s an importrnt issue to prevent the oxidation damage. Recently there are so many research about if there are any substrates can act as antioxidant in the plants. Genistein is one kind of isoflavones, and can act as antioxidant. We hypothesized that genistein one of isoflavones can protect salivary gland cells from ROS attack. Specific aim : investigate whether genistein can protect the salivary acinar cell, reduce the oxidation damage, in order to provide a possible treatment for patients with xerostomia. Method: salivary gland cells were acquired from 1-week-old mice, after 3~10 passages cells were pretreated with genistein for 24 hours, then treated with hydrogen peroxide for 0~48 hours. MTT assay, alpha-amylase assay and Immunofluorescence were used to evaluation the result. Result: Accroding to MTT assay, alpha-amylase assay, and Immunofluorescence evaluation. It looks like that with low dosage of genistein (1.25⼚3;g/ml), it can protect the acinar cells, reduce the damage from R.O.S. Conclusion: genistein is a good antioxidant, it can protect salivary gland aconar cells, reduce the oxidation damage from hydrogen peroxide.
Subjects
salivary gland cells
genistein
reactive oxygen specises
SDGs
Type
thesis
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