行政院國家科學委員會專題研究計畫期中進度報告:應用膜蛋白質檢測家禽白血病病毒(1/2)
Date Issued
2005
Date
2005
Author(s)
王金和
DOI
932313B002103
Abstract
There is a global need to develop a specific antigen detection method for subgroup J avian
leukosis virus (ALV-J) since the group-specific antigen detection is unable to distinguish
exogenous from endogenous viruses. In order to obtain monoclonal antibodies specific to ALV-J,
the N-terminus of the surface unit (gp85N) of the envelope protein from a Taiwanese ALV-J was
cloned in pRSET and then in a mammalian expression vector for monoclonal antibody
production in mice. After selection from hybridoma cells, two monoclonal antibodies, mAb14
and mAb22 were obtained. Both monoclonal antibodies caught specifically to ALV-J and the
expressed gp85 protein from ALV-J but not that from subgorup A avian leukosis virus by
immunodot assay. Furthermore, mAb22 was found to catch ALV-J by immunodot, Western blot,
immunoperoxidase and immunofluorescent assays. Thus, mAb22 was used for detecting ALV-J
antigen or virus in blood. The result shows that this monoclonal antibody could detect positive
sample. In conclusion, this monoclonal antibody might be useful for the diagnosis of ALV-J virus
or ALV-J-derived antigen.
Publisher
臺北市：國立臺灣大學獸醫學系暨研究所
Type
report
File(s)![Thumbnail Image]()
Loading...
Name
932313B002103.pdf
Size
52.02 KB
Format
Adobe PDF
Checksum
(MD5):ad9b289d4dd4337da0d776e4b43c389d