Molecular epidemiology of porcine reproductive and respiratory syndrome viruses isolated from 1991 to 2013 in Taiwan
Journal
Archives of Virology
Journal Volume
160
Journal Issue
11
Pages
2709-2718
Date Issued
2015
Author(s)
Deng, Ming-Chung
Huang, Tien-Shine
Chang, Chieh
Huang, Yu-Liang
Abstract
Porcine reproductive and respiratory syndrome virus (PRRSV) was first identified in Taiwan in 1991, but the genetic diversity and evolution of PRRSV has not been thoroughly investigated over the past 20 years. The aim of this study was to bridge the gap in understanding of its molecular epidemiology. A total of 31 PRRSV strains were collected and sequenced. The sequences were aligned using the MUSCLE program, and phylogenetic analysis were performed by the maximum-likelihood method and the neighbor-joining method using MEGA 5.2 software. In the early 1990s, two prototype strains, WSV and MD001 of the North American genotype, were first identified. Over the years, both viruses evolved separately. The population dynamics of PRRSV revealed that the strains of the MD001 group were predominant in Taiwan. Evolution was manifested in changes in the nsp2 and ORF5 genes. In addition, a suspected newly invading exotic strain was recovered in 2013, suggesting that international spread is still taking place and that it is affecting the population dynamics. Overall, the results provide an important basis for vaccine development for the control and prevention of PRRS. © 2015, Springer-Verlag Wien.
SDGs
Other Subjects
virus protein; animal; chemistry; classification; genetic variation; genetics; genotype; isolation and purification; molecular epidemiology; molecular genetics; open reading frame; phylogeny; pig; porcine reproductive and respiratory syndrome; Porcine reproductive and respiratory syndrome virus; sequence alignment; Taiwan; virology; virus genome; Animals; Genetic Variation; Genome, Viral; Genotype; Molecular Epidemiology; Molecular Sequence Data; Open Reading Frames; Phylogeny; Porcine Reproductive and Respiratory Syndrome; Porcine respiratory and reproductive syndrome virus; Sequence Alignment; Swine; Taiwan; Viral Proteins
Type
journal article