A single step method for purification of sulfated oligosaccharides
Resource
Glycoconjugate Journal 25 (9): 903-915
Journal
Glycoconjugate Journal
Pages
903-915
Date Issued
2008
Date
2008
Author(s)
Garenaux, Estelle
Yu, Shin-Yi
Florea, Doina
Strecker, G?rard
Khoo, Kay-Hooi
Gu?rardel, Yann
Abstract
Purifying and analysing sulfated oligosaccharides by mass spectrometry often constitutes a challenge. We present here a single step method to isolate sulfated compounds from a complex mixture of neutral and acidic oligosaccharide-alditols. The strategy relies on the exclusion of sulfated molecules from strong cation exchange resin. By testing a wide range of reduced mucin type O-glycans isolated from different biological sources, we demonstrate that this method permits, without prior chemical modification, the specific purification of sulfate-containing oligosaccharides present in any quantity from very complex mixtures of molecules. © 2008 Springer Science+Business Media, LLC.
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