A Glycoprotein Extracted from Golden Oyster Mushroom Pleurotus citrinopileatus Exhibiting Growth Inhibitory Effect against U937 Leukemia Cells
Resource
Journal of Agricultural and Food Chemistry, 57(15), 6706-6711
Journal
Journal of Agricultural and Food Chemistry
Pages
6706-6711
Date Issued
2009
Date
2009
Author(s)
Chen, Jian-Nan
Wang, Yuh-Tai
Wu, James Swi-Bea
Abstract
Mushrooms have become popular sources of natural antitumor, antiviral, antibacterial, antioxidative, and immunomodulatory agents. Golden oyster mushroom, Pleurotus citrinopileatus , is a common mushroom in oriental countries for human consumption. We isolated a functional protein (PCP-3A) from the fresh fruiting body of this mushroom. The isolation procedure included ammonium sulfate fractionation, DEAE-Sepharose CL-6B ion exchange chromatography, and Sephacryl S-300 gel filtration. Electrophoresis demonstrated that PCP-3A is a glycoprotein composed of 10 subunits, each approximately 45.0 kDa in size. In vitro cell study showed that PCP-3A at a concentration about 12.5 microg/mL inhibits the proliferation of human tumor cell line U937, in a time- dependent manner (24, 48, and 72 h). It failed to agglutinate rabbit and human erythrocytes, excluding its possibility from being a lectin. Flow cytometry revealed that it is capable of inhibiting the growth of U937 cells by way of S phase arrest and apoptotic induction. We suggest that PCP-3A is worth further investigating for antitumor use.
SDGs
Type
journal article
File(s)![Thumbnail Image]()
Loading...
Name
14.pdf
Size
23.38 KB
Format
Adobe PDF
Checksum
(MD5):6ebff09fa830ee772e88d70fd8dae57b