愛滋病毒Vpr 蛋白之功能研究及其與HAX-1 和脊椎肌肉萎縮基因之作用(2/3)
Date Issued
2003
Date
2003
Author(s)
黃立民
DOI
912314B002153
Abstract
HIV-1 Vpr, a 96-amino-acid 14-kDa protein, has several important and
interesting functions, including nuclear translocation of the pre-integration complex,
cell cycle arrest at the G2/M phase, transactivation, enhancement of virus replication,
and apoptosis. To understand the role of Vpr in HIV-1 life cycle and pathogenesis,
yeast two-hybrid system had been used and cDNA encoding HAX-1 or SMN1 protein
was identified. Interaction between Vpr and HAX-1 or SMN1 was characterized by in
vitro protein-protein binding assay. With the aid of a panel of Vpr deletion and point
5
mutants, we have determined the domains of Vpr involved in the interaction with
HAX-1 or SMN1. Over expressed SMN alter the localization of GFP-Vpr fusion
protein within HeLa cells, indicating SMN play unknown role in Vpr biological
function. Previously studies show that SMN modulate neuron cell apoptosis, but little
is known about SMN1 how to cause the degeneration of motor neuron the developing
to spinal muscular atrophy disease (SMA). Other studies find that Vpr induces
apoptosis in neuronal cells, and the correlations between SMN1 and antiapoptosis
protein, NAIP (neuronal apoptosis inhibitory protein) and Bcl-2, has been proved. In
coming project we will try to verify the possible functional correlation between Vpr
and SMN that involved in apoptosis pathway.
This article provides guidance for report writing under the Grant of National Science Council
beginning from fiscal year 1998.
Subjects
HIV-1
yeast two-hybrid system
SMN
Publisher
臺北市：國立臺灣大學醫學院小兒科
Type
report
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