Functional cooperation between FACT and the MCM helicase complex facilitates chromatin DNA replication
Date Issued
2005-06-08
Date
2005-06-08
Author(s)
呂勝春
DOI
932320B002105
Abstract
Chromatin is suppressive in nature to cellular enzymes that metabolize DNA, mainly
due to the inherent inaccessibility of the DNA template. Despite extensive
understanding of the involvement of chromatin-modifying factors in transcription,
role of related activities in DNA replication remains largely elusive. Here we show
that the transcription elongation factor FACT is functionally linked to DNA synthesis
progression. Its involvement in DNA replication is partly mediated by the stable
association with the replicative helicase complex, MCM, and further by the
coexistence with MCM on replication origin. Furthermore, reliant on its
nucleosome-reorganizing activity, FACT possesses the ability to facilitate chromatin
unwinding by the MCM complex, which is otherwise inert on the nucleosomal
template. As a consequence, FACT positively regulates DNA replication and S phase
progression in vivo, and such replicative role is evolutionarily conserved. Together,
our findings identify FACT as an integral and conserved component of the
endogenous replication machinery, and further outline a model in which the concerted
action of helicase and chromatin-modifying activities promotes chromatin replication.
Publisher
臺北市：國立臺灣大學醫學院分子醫學研究所
Type
journal article
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