Autoantibodies against human epithelial cells and endothelial cells after severe acute respiratory syndrome (SARS)-associated coronavirus infection
Journal
Journal of Medical Virology
Journal Volume
77
Journal Issue
1
Pages
1-7
Date Issued
2005
Author(s)
Abstract
The severe acute respiratory syndrome (SARS) is caused by infection with the SARS-associated coronavirus (SARS-CoV) and characterized by severe pulmonary inflammation and fibrosis. In this study, the development of autoantibodies against human epithelial cells and endothelial cells in patients with SARS at different time periods (the first week: phase I, 1 month after the disease onset: phase II/phase III) were investigated. Antibodies in sera of patients and healthy controls against: (1) A549 human pulmonary epithelial cell-line, (2) human umbilical venous endothelial cells (HUVEC), (3) primary human pulmonary endothelial cells (HPEC) were detected by cell-based ELISA and indirect immunofluorescence staining. The results revealed that serum levels of IgG anti-A549 cells antibodies, IgG anti-HUVEC antibodies, and IgM anti-HPEC antibodies were significantly higher in SARS patients at phase II/phase III than those in healthy controls. Sera from SARS patients at phase II/phase III could mediate complement dependent cytotoxicity against some A549 cells and HPEC. It is concluded that some autoantibodies against human epithelial cells and endothelial cells would be developed after SARS-CoV infection and this phenomenon may indicate post-infectious cellular injury and also induce SARS-induced immunopathology. ? 2005 Wiley-Liss, Inc.
SDGs
Other Subjects
autoantibody; immunoglobulin G antibody; immunoglobulin M antibody; antibody detection; article; complement system; controlled study; cytotoxicity; endothelium cell; human; human cell; immunopathology; lung alveolus epithelium; lung fibrosis; pneumonia; SARS coronavirus; severe acute respiratory syndrome; umbilical vein; Adult; Antibodies, Viral; Autoantibodies; Cell Line; Endothelial Cells; Enzyme-Linked Immunosorbent Assay; Epithelial Cells; Humans; Immunoglobulin A; Immunoglobulin G; Immunoglobulin M; Nucleocapsid Proteins; SARS Virus; Severe Acute Respiratory Syndrome; Coronavirus; SARS associated coronavirus; SARS CoV
Type
journal article