The Role of Galectin-1 and Galectin-3 in Development and Function of Plasmacytoid Dendritic Cells
Date Issued
2014
Date
2014
Author(s)
Lu, Hsueh-Han
Abstract
Galectins are animal lectins that can bind to β-galactosides. In the immune system, galectin-1 and galectin-3 have been shown to modulate immune responses in various types of immune cell. However, the role of galectin-1 and galectin-3 in the development and the function of plasmacytoid dendritic cell (pDC) has not yet been studied. pDCs produce type-1-interferon (IFN-I) in response to viral infection and then enhance antiviral activities. Thus, we examined the effect of galectin-1 and galectin-3 on pDC development and IFN-I production by pDCs upon the stimulation of R848 or CpG through TLR7/9. In preliminary results, we show that galectin-1 and galectin-3 didn’t influence the development of pDC in vitro or in vivo. In addition, we found that CpG or R848-induced mRNA levels of IFN-I (ifnα, ifnα4, ifnβ) and interferon regulatory factor (IRF7) were significantly higher in gal-3-/- Flt3L-BMDCs and in the contrast, significantly lower in gal-1-/- Flt3L-BMDCs when compared to that in WT Flt3L-BMDCs. Moreover, at the protein level, the production of IFNα by gal-3-/- Flt3L-BMDCs stimulated with CpG or R848 is higher than WT Flt3L-BMDCs. And, the production of IFNα by gal-3-/- Flt3L-BMDCs is lower than WT Flt3L-BMDCs. Our data suggested that galectin-3 may negatively regulate the function of pDCs, on the other hand that galectin-1 may positively regulate the function of pDC. However, the mechanism of how galectin-3 and galectin-1 involved in IFN-I production need to be further explored.
Subjects
漿狀樹突細胞
半乳糖凝集素-1
半乳糖凝集素-3
第一型干擾素
類鐸受體7/9
Type
thesis
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