Highly conserved influenza A virus epitope sequences as candidates of H3N2 flu vaccine targets
Journal
Genomics
Journal Volume
100
Journal Issue
2
Pages
102-109
Date Issued
2012
Abstract
This study focused on identifying the conserved epitopes in a single subtype A (H3N2)-as candidates for vaccine targets. We identified a total of 32 conserved epitopes in four viral proteins [22 HA, 4PB1, 3 NA, 3 NP]. Evaluation of conserved epitopes in coverage during 1968-2010 revealed that (1) 12 HA conserved epitopes were highly present in the circulating viruses; (2) the remaining 10 HA conserved epitopes appeared with lower percentage but a significantly increasing trend after 1989 [p < 0.001]; and (3) the conserved epitopes in NA, NP and PB1 are also highly frequent in wild-type viruses. These conserved epitopes also covered an extremely high percentage of the 16 vaccine strains during the 42. year period. The identification of highly conserved epitopes using our approach can also be applied to develop broad-spectrum vaccines. ? 2012 Elsevier Inc.
Subjects
Bioinformatics; Broad-spectrum vaccine; Epitope; Influenza virus
SDGs
Other Subjects
epitope; influenza vaccine; nonstructural protein 1; nonstructural protein 2; protein m1; protein M2; protein NA; protein NP; protein PA; protein PB1; protein pb1 f2; protein PB2; unclassified drug; virus hemagglutinin; virus protein; amino acid sequence; antigen detection; article; B lymphocyte; CD4+ T lymphocyte; CD8+ T lymphocyte; drug targeting; gene cluster; genetic conservation; influenza A (H2N2); influenza vaccination; Influenza virus A H2N2; microbial diversity; nonhuman; priority journal; protein targeting; sequence homology; virus strain; wild type; Cluster Analysis; Epitopes; Humans; Influenza A Virus, H3N2 Subtype; Influenza Vaccines; Influenza, Human; Sequence Analysis; Viral Proteins; flu vaccine; Influenza A virus; Orthomyxoviridae
Type
journal article