Frequent Genetic Alterations at the Distal Region of Chromosome lp in Human Hepatocellular Carcinomas
Journal
Cancer Research
Journal Volume
54
Journal Issue
15
Pages
4188-4192
Date Issued
1994
Author(s)
Abstract
Cytogenetic analysis of hepatocellular carcinoma (HCC) cell lines and primary HCC tissues has demonstrated chromosome 1p to be the region most commonly affected. To refine the altered locus, genetic abnormalities of this region were surveyed systemicaily by microsatellite polymorphism analysis. Twelve sets of primers evenly distributed on chromosome lp which can amplify di- or tetranucleotide repeat length polymorphism by polymerase chain reaction were selected. The results were then supplemented by the conventional restriction fragment length polymorphism study. A comparison of the allele patterns between 30 pairs of HCC and their corresponding nontumor DNAs discovered chromosome 1p aberrations in 15 of 30 tumors (50%). The abnormalities can be classified into three groups. The first aberration was typical loss of heterozygosity that was found in 9 HCCs (30%). The second aberration was a 2-3-fold increase of allelic dosage, which was detected in 6 HCCs (20%). The third aberration was the novel microsatellite polymorphism, which was detected in 3 cases (10%). These abnormalities seemed to cluster at the distal part of chromosome 1p, with a common region mapped to 1p35-36, which is also the region with frequent loss of heterozygosity in neuroblastoma and colorectal and breast cancers. Therefore, loss of putative tumor suppressor gene(s) in this locus may participate in the development of hepatocellular carcinoma and a wide range of human cancers. ? 1994, American Association for Cancer Research. All rights reserved.
SDGs
Other Subjects
article; chromosome 1p; chromosome aberration; chromosome analysis; clinical article; controlled study; gene loss; heterozygosity; human; human tissue; liver cell carcinoma; polymerase chain reaction; priority journal; tumor suppressor gene; Carcinoma, Hepatocellular; Chromosome Deletion; Chromosome Mapping; Chromosomes, Human, Pair 1; DNA, Satellite; Human; Liver Neoplasms; Polymerase Chain Reaction; Polymorphism (Genetics); Polymorphism, Restriction Fragment Length; Repetitive Sequences, Nucleic Acid; Support, Non-U.S. Gov't
Type
journal article