Effect of the ethanolic extracts from lotus leaf on the early stages of atherosclerosis
Date Issued
2004
Date
2004
Author(s)
Lee, Chi-Ying
DOI
zh-TW
Abstract
動脈粥狀硬化除了是一慢性發炎疾病外，亦是一不能以單一病因來探討之疾病，早期研究認為動脈粥狀硬化和血管表面脂質的堆積有密切關係，而近年來越來越多的研究指出氧化壓力和低密度脂蛋白的氧化也是主要的病因之一。因此本研究擬從氧化傷害之角度出發，利用試管試驗及細胞模式探討荷葉乙醇萃取物及其四種溶劑（正己烷、乙酸乙酯、正丁醇及水）區分層對動脈粥狀硬化早期發展的影響，並期望能得知其有效區分層及可能活性物質。
實驗結果顯示，荷葉乙醇萃取物之乙酸乙酯區分層具有最佳的清除活性氧屬自由基能力，且能有效的抑制銅離子誘導人體離體低密度脂蛋白氧化，顯示乙酸乙酯區分層可能具有降低氧化壓力以防止動脈粥狀硬化形成之能力；同時乙酸乙酯區分層亦可促進巨噬細胞吞噬氧化低密度脂蛋白，且能減緩巨噬細胞衍生的泡沫細胞及氧化低密度脂蛋白所誘發之發炎反應，顯示乙酸乙酯區分層可能對動脈粥狀硬化早期發展具影響能力，並藉此達到延緩動脈粥狀硬化發展之效用。
第二部分則是針對乙酸乙酯區分層進行膠體層析後得1 ~ 11個次區分層進行試驗，其中第8、9、10層於各試驗所表現出來的能力皆較乙酸乙酯區分層來的好，於是將此三層進行HPLC比對後，發現其中可能的有效活性化合物為quercetin、p-coumaric acid、syringic acid或其他類黃酮類物質，因此推測荷葉中具延緩動脈粥狀硬化發展之能力可能有一部分來自於類黃酮及酚酸類物質，另一部分可能來自於未定性出的其他類黃酮物質或生物鹼。
Atherosclerosis is a chronic inflammation disease that cannot be defined as a single disease entity. In early study, it shows atherosclerosis has great relationship with lipid accumulated in surface of vessel but recently research has indicated oxidative stress and oxidation of LDL(low density lipoprotein)being major causes, too. In this research, we use the ethanolic extract from lotus leaf(LLE)and its four partitions(LLEP1、LLEP2、LLEP3、LLEP4)as raw materials to study their effects on the early stages of atherosclerosis by in vitro test and cell model.
The results showed that partition of ethyl acetate from LLE(LLEP2)had the greatest scavenging ability of reactive oxygen species and inhibition of LDL oxidation. LLEP2 could also promote macrophage ability to endocyte oxLDL and to decrease the inflammation which was induced by oxLDL and macrophage-derived foam cells. Consequently, it seems LLEP2 can affect the early stages to retard the formation of atherosclerosis.
In the next part assay, we used LLEP2 as materials to do gel filtration and got 11 subfractions(1 ~ 11). According the results we found subfraction of 8, 9, 10 had better ability than that of LLEP2. The results of HPLC showed the possible active compounds in the subfraction of 8, 9, 10 were quercetin、p-coumaric acid、syringic acid and other flavonoids. The ability of lotus leaf to retard the formation of atherosclerosis could be from flavonoids and some unknown compounds.
Subjects
動脈粥狀硬化
氧化壓力
荷葉
lotus leaf
atherosclerosis
oxidative stress
Type
thesis
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