Effects of aeration on tropane alkaloid production by transformed root of Atropa belladonna in flask cultures.
Resource
Ferment Bioeng ( { אּ J Biosci Bioeng),86,614-616.
Journal
Ferment Bioeng ( { אּ J Biosci Bioeng)
Journal Issue
86
Pages
614-616
Date Issued
1998-01
Date
1998-01
Author(s)
Abstract
Transformed roots of Atropa belladonna strain M8 were cultured in shaking flasks aerated with normal air at 1 vvm, capped with aluminum foil, or capped with a silicon rubber stopper, to investigate the effects of aeration on root growth and on tropane alkaloid production and composition. After four weeks, the tropane alkaloid productivity of roots grown in flasks aerated at 1 vvm reached 52.9 mg/l, and the individual tropane alkaloid contents in the roots were, in mg/l dry wt., (-)-hyoscyamine 4.8, 6β-hydroxyhyoscyamine 1.4, littorine 1.2, and scopolamine 1.0. These results indicate that aeration at 1 vvm air has an important effect on the control of the tropane alkaloid composition in root cultures. In particular, the scopolamine ratio increased from 5.7 to 11.9% of the total alkaloids accumulated in the belladonna cultured roots. Transformed roots of Atropa belladonna strain M8 were cultured in shaking flasks aerated with normal air at 1 vvm, capped with aluminum foil, or capped with a silicon rubber stopper, to investigate the effects of aeration on root growth and on tropane alkaloid production and composition. After four weeks, the tropane alkaloid productivity of roots grown in flasks aerated at 1 vvm reached 52.9 rag/l, and the individual tropane alkaloid contents in the roots were, in mg/l dry wt., (-)-hyoscyamine 4.8, 6β- hydroxyhyoscyamine 1.4, littorine 1.2, and scopolamine 1.0. These results indicate that aeration at 1 vvm air has an important effect on the control of the tropane alkaloid composition in root cultures. In particular, the scopolamine ratio increased from 5.7 to 11.9% of the total alkaloids accumulated in the belladonna cultured roots.