The development of poly-silicon nanowire biosensors
Date Issued
2010
Date
2010
Author(s)
Wang, Jui-Ching
Abstract
In recent years, the medical diagnostics and diagnostic judgments begin using molecular markers approach, and highly specific therapies aimed at molecular targets. Therefore we need high-throughput methods for rapid detection of biological molecules. Expect to achieve early detection of disease, early treatment goals. The silicon nanowire transistor devices had been proven as high sensitivity biomolecular sensors. The silicon nanowire devices can detect biomolecules with high sensitivity, real-time, fast and accurate.
In this work, we fabricated poly-silicon nanowire thin-film transistors with simple and fast method. The shifts of threshold voltage and the changes of current were detected by exposing the poly-silicon nanowires to buffer solutions of different pH value. A biomolecular detection experiment was set up using the specific binding of biotin to streptavidin/avidin. First we immobilized protein with fluorescent molecules on the poly-silicon surface, and used fluorescent microscope to observe the fluorescent pattern. We measured the electronic properties of the poly-silicon nanowire thin-film transistors in different concentration streptavidin/avidin. Specific electric changes were observed for streptavidin and avidin sensing when the poly-silicon nanowire surface was modified with biotin and streptavidin at pM to dozens nM range could be distinguished.
Subjects
poly-silicon
nanowire
biosensor
Type
thesis
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