Antiobesity effect of Lactobacillus reuteri 263 associated with energy metabolism remodeling of white adipose tissue in high-energy-diet-fed rats
Journal
Journal of Nutritional Biochemistry
Journal Volume
54
Pages
87-94
ISSN
1873-4847
Date Issued
2018-04
Author(s)
Abstract
Obesity is a serious and costly issue to the medical welfare worldwide. Probiotics have been suggested as one of the candidates to resolve the obesity-associated problems, but how they combat obesity is not fully understood. Herein, we investigated the effects of Lactobacillus reuteri 263 (L. reuteri 263) on antiobesity using four groups of Sprague–Dawley rats (n=10/group), namely, C (normal diet with vehicle treatment), HE [high-energy diet (HED) with vehicle treatment], 1X (HED with 2.1×10 9 CFU/kg/day of L. reuteri 263) and 5X (HED with 1.05×10 10 CFU/kg/day of L. reuteri 263), for 8 weeks. L. reuteri 263 improved the phenomenon of obesity, serum levels of proinflammatory factors and antioxidant enzymes. More importantly, L. reuteri 263 increased oxygen consumption in white adipose tissue (WAT). The mRNA expressions of thermogenesis genes uncoupling protein-1, uncoupling protein-3, carnitine palmitoyltransferase-1 and cell death-inducing DFFA-like effector-a were up-regulated in WAT of the 5X group. Moreover, L. reuteri 263 might induce browning of WAT due to the higher mRNA levels of browning-related genes peroxisome proliferator-activated receptor-γ, PR domain containing-16, Pparγ coactivator-1α, bone morphogenetic protein-7 and fibroblast growth factor-21 in the 1X and 5X groups compared to the HE group. Finally, L. reuteri 263 altered the expressions of genes involved in glucose and lipid metabolisms in WAT, including increasing the levels of glucose transporter type 4 and carbohydrate-responsive element-binding protein and decreasing the expression of Acetyl-CoA carboxylase-1. The results suggest that L. reuteri 263 may treat obesity through energy metabolism remodeling of WAT in the high-energy-diet-induced obese rats.
Subjects
Antiobesity
Browning of white adipose tissue
Energy metabolism remodeling
Lactobacillus reuteri 263
Probiotics
SDGs
Other Subjects
acetyl coenzyme A carboxylase; adiponectin; carbohydrate responsive element binding protein; carnitine palmitoyltransferase I; cell death inducing DFFA like effector alpha; cholesterol; fibroblast growth factor 21; glucose; glucose transporter 4; interleukin 6; low density lipoprotein; messenger RNA; osteogenic protein 1; oxygen; peroxisome proliferator activated receptor gamma; peroxisome proliferator activated receptor gamma coactivator 1alpha; PR domain containing 16 protein; protein; triacylglycerol; tumor necrosis factor; unclassified drug; uncoupling protein 1; uncoupling protein 3; antiobesity agent; antioxidant; enzyme; glucose; probiotic agent; animal experiment; animal model; Article; body fat; caloric intake; cell respiration; cholesterol blood level; colony forming unit; comparative study; controlled study; energy expenditure; energy metabolism; fat pad; food intake; glucose blood level; Lactobacillus reuteri; lipid metabolism; lipid storage; lipoprotein blood level; male; mesenteric fat; nonhuman; obesity; oxygen consumption; rat; triacylglycerol blood level; upregulation; white adipose tissue; animal; blood; energy metabolism; gene expression regulation; genetics; metabolism; oral drug administration; physiology; Sprague Dawley rat; white adipose tissue; Adipose Tissue, White; Administration, Oral; Animals; Anti-Obesity Agents; Antioxidants; Energy Intake; Energy Metabolism; Enzymes; Gene Expression Regulation; Glucose; Lactobacillus reuteri; Lipid Metabolism; Male; Oxygen Consumption; Probiotics; Rats, Sprague-Dawley
Publisher
Elsevier Inc.
Type
journal article