Antioxidant effect of the hydrolysate of Aluterus monoceros skin on fibroblasts and keratinocytes
Date Issued
2008
Date
2008
Author(s)
Chen, Yi-Ju
Abstract
Currently, with the popularity of antiaging notion, the physiological changes of aged skin have become one of the main issues concerned by the public. Driven by rising consumer interest and demand for natural products, more and more natural raw materials have been investigated for the improvement of skin aging. The purpose of this research was to investigate the skin care potential of the products prepared by enzymatic hydrolysis or fermentation of the skin of Aluterus monoceros. This study was divided into two parts. The first part was the evaluation of DPPH free radical scavenging activity and the stimulating effect on collagen synthesis of fermented and enzyme hydrolyzed products of the skin of Aluterus monoceros. The results revealed B. subtilis natto-fermented fish skin products had higher DPPH free radical scavenging activity than other preparations. he second part of this research was to find the optimal fermentation condition in 6.6 L fermentor for B. subtilis natto-fermented fish skin products on antioxidant activities and intracellular reactive oxygen species (ROS) scavenging effects induced by hydrogen peroxide and solar simulator in human skin fibroblasts and keratinocytes. It was found that fermented products, in comparison with unfermented samples showed a dramatic increase in DPPH, ABTS+, superoxide anion and hydrogen peroxide scavenging effects. The products fermented at 1.5 vvm for 72 h presented the best DPPH free radical scavenging effect, while the products fermented at 0.5 vvm for the same fermentation time appeared to have the best ABTS+, superoxide anion, and hydrogen peroxide scavenging effects. In addition, the growth rate of fibroblast treated with the products fermented at 1vvm for 24 h increased 16%, at the concentration of 1 mg/mL. Further, the products fermented at 0.5 vvm for 72 h significantly decreased the amount of hydrogen peroxide- and solar simulator- induced the accumulation of ROS in human skin fibroblasts and keratinocytes.aken together, it is cleared that the products fermented at 0.5 vvm for 72 h had free radical scavenging activities and intracellular ROS- scavenging effects on human skin fibroblasts and keratinocytes. Therefore, we expect that B. subtilis natto-fermented fish skin products might be a promising candidate for eliminating the age-dependent accumulation of ROS in human skin cells, and have a potential use for skincare application.
Subjects
Aluterus monoceros skin
Bacillus subtilis natto
fermentation
antioxidation
fibroblasts
keratinocytes
Type
thesis
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