Glycomic mapping of pseudomucinous human ovarian cyst glycoproteins: Identification of Lewis and sialyl Lewis glycotopes
Resource
PROTEOMICS 7 (20): 3699-3717
Journal
PROTEOMICS
Pages
3699-3717
Date Issued
2007
Date
2007
Author(s)
Wu, Albert M.
Khoo, Kay-Hooi
Yu, Shin-Yi
Yang, Zhangung
Kannagi, Reiji
Watkins, Winifred M.
Abstract
Expression of sialyl Lewis x (sLex) and sialyl Lewis a (sLeaa) on cell-surface glycoproteins endows cells with the ability to adhere to E-, P-, and L-selectins present on endothelia, platelets, or leukocytes. Special arrangements of these glycotopes in cancers are thought to play a key role in metastasis. Previous studies have mostly described membrane-bound sLex and sLea activities. In this report, the major O-glycans of the secreted human ovarian cyst sialoglycoproteins from a Le(a+) nonsecretor individual (human ovarian cyst sample 350) were characterized by MS/MS analyses and immuno-/lectin-chemical assays. The results showed that HOC 350 carries a large number of epitopes for sLex, sLea, and Lea reactive antibodies. Advanced MS/MS sequencing coupled with mild periodate oxidation and exoglycosidase digestions further revealed that the O-glycans from HOC 350 are mostly of core 1 and 2 structures, extended and branched on the 3-arm with both type I and type II chains, complete with variable degrees of terminal sialylation and/or fucosylation to yield the sLex or sLea epitopes. Thus, the underlying core and peripheral backbone structures are similar to that of a previously proposed composite structural model for nonsialylated human ovarian cysts O-glycans, but with some notable distinguishing structural features in addition to sialylation. ? 2007 Wiley-VCH Verlag GmbH & Co. KGaA.
Subjects
Glycomics; Human ovarian cyst glycoproteins; Sialoglycoprotein; Sialyl Lewis a; Sialyl Lewis x
SDGs
Other Subjects
glycan derivative; glycoprotein; periodate; sialoglycoprotein; sialyl Lewis x antigen; article; controlled study; glycobiology; human; human tissue; mass spectrometry; ovary cyst; oxidation; priority journal; protein analysis; protein secretion; Antigens, Tumor-Associated, Carbohydrate; Carbohydrate Sequence; Epitopes; Female; Gangliosides; Humans; Lewis Blood-Group System; Molecular Sequence Data; Oligosaccharides; Ovarian Cysts; Polysaccharides; Sialoglycoproteins; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Tumor Markers, Biological