Differential expression and functional characterization of the NADPH cytochrome P450 reductase genes from Nothapodytes foetida
Journal
Plant Science
Journal Volume
190
Pages
16-23
Date Issued
2012
Author(s)
Abstract
Three unique NADPH:cytochrome P450 reductase (CPR) cDNAs have been isolated from a . Nothapodytes foetida cDNA library and characterized. Phylogenetic analysis showed that NfCPR1 is a class I isoform, whereas NfCPR2 and NfCPR3 are class II isoforms. Both . NfCPR1 and . NfCPR2 transcripts were detected in all examined organs of . N. foetida, with the highest level for . NfCPR1 being in the seeds whereas for . NfCPR2 predominantly in leaves. In contrast, . NfCPR3 transcripts were only detected in flower buds and seeds at almost equal expression levels. Moreover, . NfCPR1 expression did not change during wounding treatment, whereas . NfCPR2 and . NfCPR3 were induced in response to wounding. Microsomes isolated from insect cells co-expressing NfCPR2 and cytochrome P450 enzyme geraniol 10-hydroxylase (G10H) enhanced the production of eriodictyol from naringenin approximately 11-fold relative to control G10H-only insect cells, indicating the supportive role of NfCPR2 for G10H activity in insect cells. © 2012 Elsevier Ireland Ltd.
Type
journal article