Magnetic cryopreservation for dental pulp stem cells
Journal
Cells Tissues Organs
Journal Volume
196
Journal Issue
1
Pages
23-33
Date Issued
2012
Author(s)
Abstract
Magnetic cryopreservation has been successfully used for tooth banking with satisfactory implantation outcomes, suggesting that the method preserves human periodontal ligament cells and dental pulp stem cells (DPSCs). Therefore, magnetic cryopreservation may be applied for the preservation of DPSCs; however, this method has not been evaluated yet. A reliable cryopreservation method for live-cell preservation is important for the clinical applications of regenerative medicine. The conventional slow-freezing procedure with 10% dimethylsulfoxide (DMSO) may not be appropriate for stem cell-based therapies because DMSO is cytotoxic. The objective of this study was to investigate whether magnetic cryopreservation can be applied for DPSC cryopreservation. Cells isolated from human dental pulp were subjected to magnetic cryopreservation. Postthawing cell viability, adhesion, proliferation, expression of markers for mesenchymal stem cells (MSCs), differentiation ability of magnetically cryopreserved DPSCs and DNA stability were compared to those of cells subjected to the conventional slow-freezing method. The results indicated that a serum-free cryopreservation medium (SFM) containing 3% DMSO is optimal for magnetic cryopreservation. Post-thaw magnetically cryopreserved DPSCs express MSC markers, and perform osteogenesis and adipogenesis after induction similarly to fresh MSCs. No significant DNA damage was found in magnetically cryopreserved DPSCs. Magnetic cryopreservation is thus a reliable and effective method for storage of DPSCs. The smaller amount of DMSO required in SFM for cryopreservation is beneficial for the clinical applications of post-thaw cells in regenerative medicine. Copyright ? 2012 S. Karger AG, Basel.
SDGs
Other Subjects
biological marker; dimethyl sulfoxide; adipogenesis; adult; article; bone development; cell adhesion; cell differentiation; cell proliferation; cell viability; cryopreservation; cytotoxicity; DNA damage; female; human; human cell; magnetic cryopreservation; magnetic field; male; mesenchymal stem cell; normal human; priority journal; stem cell; stem cell transplantation; thawing; tooth pulp; Adult; Apoptosis; Biological Markers; Cell Adhesion; Cell Differentiation; Cell Proliferation; Cryopreservation; Cryoprotective Agents; Dental Pulp; Dimethyl Sulfoxide; DNA; DNA Damage; Female; Freezing; Humans; Magnetics; Male; Stem Cells
Type
journal article