Effect of pulmonary epithelial lining fluid on PM-induced oxidative damage
Date Issued
2004
Date
2004
Author(s)
Cheng, Yi-Ling
DOI
zh-TW
Abstract
Previous studies have reported that exposure to particulate matter（PM）induces oxidative stress and respiratory tract inflammation. The component of PM responsible for this health effect remained unclear. Pulmonary epithelial lining fluid（ELF）consists of various antioxidants, which can protect lung cells against the oxidative damage of PM. However the exact mechanism remains unclear. The aim of this study was to determine the effect of ELF on PM-induced oxidative damage.
This study was conducted with and without epithelial cells. We also use synthetic human epithelial lining fluids (ELF, Guobin et al., 1999.) in both cell and cell free system. In cell free system, total ROS was measured with DCFH assay after exposure to ultrafine carbon black (ufCB; at 0, 50, 150ug/ml), transitional metal (FeSO4; at 0,100, 500uM) and organic component（DEP extract; at 0, 25, 50ug/ml）. Subsequently, we established an in vitro transwells exposure system consisting with or without of ELF. A549 cells were exposed to particles for four hours. ROS was then measured using DCFH assay and DNA breakage was determined by single-cell gel electrophoreses (Comet assay).
The results indicated that in cell free system, the amounts of ROS increased with exposure concentration and exposure time. ELF significantly decreased ROS by 100% 、56.5% and 1% as compared to culture medium after ufCB、FeSO4 or DEP extract exposure（p<0.05）. Furthermore, in cell system, ROS decreased 91%、 83%and 1% with the treatment of ELF compared to culture medium after cells exposed to ufCB 、FeSO4 and DEP extract. ELF also can decrease DNA single-strand breakage. Furthermore, there was no difference on IL-8 secretion after exposure to different componemts.
Our results suggest that ELF can decrease total ROS induced by ultrafine carbon black、FeSO4 and DEP extract. Therefore ELF can protect A549 cells from oxidative damage.
Subjects
肺部上皮內襯液體
反應性氧化物種
大氣微粒
Reactive oxygen species (ROS)
Epithelial lining fluid（ELF）
Particulate matter（PM）
SDGs
Type
thesis
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