Correlation of expression of CD44 isoforms and E-cadherin with differentiation in human urothelial cell lines and transitional cell carcinoma
Journal
Cancer Letters
Journal Volume
89
Journal Issue
1
Pages
81-87
Date Issued
1995
Author(s)
Abstract
Using immunostaining, immunoblot, reverse-transcriptase polymerase chain reaction and Southern blot, we found that expressions of CD44 isoforms and E-cadherin were very closely linked and were correlated with the differentiation status in human urothelial cell lines and clinical specimens of transitional cell carcinoma. Normal urothelium, well to moderately differentiated cell lines and surgical samples expressed E-cadherin and large CD44 isoforms containing exon v6, which was pivotal in metastasis of rat pancreatic cell line model. Poorly differentiated cell lines and surgical samples, were E-cadherin-negative and expressed primarily standard form CD44, which did not contain exon v6. We concluded that CD44v6 isoforms and E-cadherin were both down-regulated during the carcinogenesis of urothelium. The large exon v6 containing CD44 isoforms were readily detected in normal urothelium, therefore, were not likely linked to cancer metastasis. E-cadherin and CD44v6 may be used as differentiation markers for human urothelial tumors. Immunohistochemical study solely with antibody against epitopes encoded by exon v6 alone is not informative enough as other alternatively spliced exons may change the function of CD44v6 isoforms. ? 1995.
SDGs
Other Subjects
hermes antigen; monoclonal antibody; uvomorulin; alternative rna splicing; animal cell; article; carcinogenesis; cell differentiation; exon; human; human cell; human tissue; immunoblotting; immunohistochemistry; metastasis; nonhuman; pancreas cell; polymerase chain reaction; priority journal; rat; southern blotting; transitional cell carcinoma; urogenital tract tumor; Animal; Antigens, CD44; Base Sequence; Bladder; Bladder Neoplasms; Blotting, Southern; Cadherins; Carcinoma, Transitional Cell; Carrier Proteins; Cell Differentiation; Cells, Cultured; Comparative Study; Down-Regulation; Exons; Human; Immunoblotting; Isomerism; Mice; Mice, Nude; Molecular Sequence Data; Polymerase Chain Reaction; Receptors, Cell Surface; Receptors, Lymphocyte Homing; Support, Non-U.S. Gov't; Animalia
Type
journal article