The Effects of Rice Diets on the Antioxidant Capacity of Liver and Colon Tissues in C57BL/6JNarl and ICR Mice
Date Issued
2010
Date
2010
Author(s)
Lin, Yuen-Hsin
Abstract
Rice has been the staple food and an important cash crop for Asians, including Taiwanese for a long time. There are abundant antioxidants and anti-inflammation substances in rice, most of them exist in the bran layer and the germ. According to the epidemiological studies, the more intake of those foods containing antioxidant substances, the less morbidity and mortality of human diseases. Therefore, replacing the polished rice with the whole grain rice diet should be a pro-health dietary pattern. Different from other studies using extracts from rice bran, this study used rice diet to investigate the effects on the antioxidant capacity of liver and colon tissue of mice. Forty-five male inbred C57BL/6JNarl mice were obtained from the National Laboratory Animal Center and forty-five male outbred ICR mice were obtained from the BioLasco Taiwan Co., Ltd. Mice were assigned to 5 dietary groups: an AIN-93G group (93G), a modified high protein AIN-93G group (M93G), a chow diet group (Chow), a polished rice high protein group (PR), and a bran-enriched brown rice high protein group (RB). The mice were fed with the experimental diets and the body weight and food intake were recorded routinely during the experiment. Mice were sacrificed after 4-week fed with experimental diets. Liver tissues were collected for analysis of the antioxidant related enzymes, such as glutathione peroxidase (GPx), glutathione reductase (GR), glutathione S-transferase (GST), superoxide dismutase (SOD) and catalase (CAT). Colon tissues were collected for analysis of glutathione (GSH) and the antioxidant enzymes, and immunochemistry assays of cell proliferation marker, Ki-67.
The results show the 2 mouse strains had different responses to the same rearing condition. The body weight of male C57BL/6JNarl mice had obvious difference, but most of the liver antioxidant enzymes had no significant differences. However, male ICR mice had converse results. The cell proliferation capacity in the mucosa epithelial cells of distal colon had a similar trend: the 93G group and M93G group had lower Ki-67 expression. The mice of both strains fed with the PR and RB diet were heavier than others. Chow diet and RB diet enhanced liver antioxidant enzymes specific activity of the 2 mouse strains, and so did in the proximal colon of male ICR mice. In conclusion, these results indicate rice diets and non-refined diets can increase tissue antioxidant capacity and have a great possibility to prevent disease.
Subjects
rice diets
brown rice
rice bran
antioxidant
GSH
GPx
GR
GST
SOD
CAT
mouse strains
SDGs
Type
thesis
File(s)![Thumbnail Image]()
Loading...
Name
ntu-99-R97b47310-1.pdf
Size
23.32 KB
Format
Adobe PDF
Checksum
(MD5):bc37a009929c1153ddcd0c19a81e9e99