Infusion of porcine-derived amniotic fluid stem cells for treatment of experimental colitis in mice
Journal
Chinese Journal of Physiology
Journal Volume
60
Journal Issue
6
Pages
345-352
Date Issued
2017
Author(s)
Abstract
Recently, stem cells have offered an alternative treatment for inflammatory bowel disease (IBD) or colitis to overcome the poor outcomes associated with current therapies. Amniotic fluid-derived stem cells (AFSCs) have the potential for the regeneration of impaired organs and the recovery of normal physiologic functions of damaged tissues without ethical concerns or risk of tumor formation. In this work, we aimed to examine the therapeutic effects of infusion of porcine AFSCs (pAFSCs) in dextran sulfate sodium (DSS)-induced colitis in mice. Treatment with pAFSCs was shown to inhibit the shortening of the colon after induction of colitis and dramatically ameliorated the body weightloss induced by the DSS treatment. In addition, pAFSCs could also reduce the extent of the inflamed area represented by epithelial mesenchymal transformation in the colitis mice. The levels of the inflammatory cytokines interleukin 6 (IL-6) and interferon gamma (IFN-γ) were also reduced in colitis mice transplanted with pAFSCs. In conclusion, pAFSCs can ameliorate experimental colitis in mice, suggesting that they may be a potential treatment for IBD or colitis. ? 2017 by The Chinese Physiological Society and Airiti Press Inc.
Subjects
Colitis mouse model; Porcine amniotic fluid-derived stem cells
SDGs
Other Subjects
dextran sulfate; gamma interferon; interleukin 6; amniotic fluid stem cell; animal cell; animal experiment; animal model; animal tissue; Article; body weight loss; clinical effectiveness; controlled study; epithelial mesenchymal transition; experimental colitis; histopathology; immunohistochemistry; mouse; nonhuman; pig; stem cell transplantation; amnion fluid; animal; colitis; cytology; disease model; embryonic stem cell; female; Institute for Cancer Research mouse; procedures; stem cell transplantation; transplantation; Amniotic Fluid; Animals; Colitis; Disease Models, Animal; Embryonic Stem Cells; Female; Mice; Mice, Inbred ICR; Stem Cell Transplantation; Swine
Type
journal article