Expression of syndecan-1 (CD138) in nasopharyngeal carcinoma is correlated with advanced stage and poor prognosis
Journal
Human Pathology
Journal Volume
37
Journal Issue
10
Pages
1279-1285
Date Issued
2006
Author(s)
Chen C.-L
Abstract
Nasopharyngeal carcinoma (NPC) is an important Epstein-Barr virus-associated head and neck malignancy in Taiwan. Syndecan-1 (CD138) is involved in growth, differentiation, invasiveness, and metastatic potential of certain tumors, but its expression in NPC has never been studied. In this study, detection of expression of syndecan-1 protein and Epstein-Barr virus-encoded latent membrane protein-1 (LMP-1) in primary, recurrent, and metastatic NPC specimens in paraffin sections was performed by immunohistochemistry. The quantity of syndecan-1 messenger RNA in tumor cells was investigated by real-time reverse transcriptase polymerase chain reaction using laser capture microdissection. The results of immunohistochemical staining of syndecan-1 and LMP-1 correlated with clinicopathologic features of NPC. Eighteen (20.9%) of 86 primary, 9 (24.3%) of 37 recurrent, and 15 (44.1%) of 34 metastatic NPC samples were positive for syndecan-1, and 37 (43.0%) primary, 18 (48.6%) recurrent, and 12 (35.3%) metastatic samples were positive for LMP-1 expression. Primary NPCs with syndecan-1 protein expression were more frequently associated with advanced clinical stages and worse 5-year survival rates than those without (P = .015 and P = .0021, respectively). Conversely, the LMP-1 expression did not correlate with tumor stage or prognosis but occurred more often in nonkeratinizing carcinoma than keratinizing squamous cell carcinoma (unpublished observation). The inverse expression of syndecan-1 and LMP-1 was noted in primary NPC specimens (total 4/18 versus 35/68, P = .05). The reverse transcriptase polymerase chain reaction revealed low syndecan-1 messenger RNA levels in both primary and metastatic NPC. In conclusion, the protein expression of syndecan-1 in 21% of primary NPC was associated with advanced disease and poor prognosis, and the protein expression correlated with transcription levels. ? 2006 Elsevier Inc. All rights reserved.
Subjects
Epstein-Barr virus; Nasopharyngeal carcinoma; Syndecan-1
SDGs
Other Subjects
latent membrane protein 1; syndecan 1; adult; advanced cancer; aged; antigen expression; article; cancer staging; cancer survival; clinical feature; controlled study; correlation analysis; female; human; human tissue; immunohistochemistry; major clinical study; male; nasopharynx carcinoma; prognosis; protein expression; reverse transcription polymerase chain reaction; squamous cell carcinoma; survival rate; Carcinoma; Gene Expression; Herpesvirus 4, Human; Humans; Immunoenzyme Techniques; Membrane Glycoproteins; Nasopharyngeal Neoplasms; Neoplasm Metastasis; Neoplasm Recurrence, Local; Neoplasm Staging; Prognosis; Proteoglycans; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; RNA, Neoplasm; Syndecan-1; Syndecans; Tumor Markers, Biological; Viral Matrix Proteins
Type
journal article