Use of a diffusion model for assessing the performance of poly(vinyl alcohol) bioartificial pancreases
Journal
Journal of Biomedical Materials Research
Journal Volume
40
Journal Issue
3
Pages
385-391
Date Issued
1998
Author(s)
Abstract
Islets of Langerhans surrounded by a semipermeable membrane to prevent an immune response by the host immunosystem is a potential way of treating type I diabetes mellitus. In this study, poly(vinyl alcohol) (PVA) tubular membranes with added polyethylene glycol to create pores in the skin layer were prepared to improve their diffusion property. In a static incubation study, islets cultured in the PVA tubular membranes still demonstrated their function of secreting insulin after 30 days. When the tubular PVA bioartificial pancreas was perifused in a small chamber with RPMI-1640 medium containing glucose at concentrations of 5.6-16.6 mmol/L, insulin release began to increase without delay. Therefore, such a membrane is an alternative potential material for a bioartificial pancreas. In addition, a mathematical mass transfer model of insulin release was developed and compared with the perifusion data. It was shown that satisfactory kinetics could be achieved with a PVA membrane. However, the model showed that the insulin output of islets cultured in the PVA tubular membrane must be increased to improve the performance significantly. These findings suggest that a bioartificial pancreas using a PVA membrane is a promising material, but the technique for seeding islets in the chamber requires further modification.Islets of Langerhans surrounded by a semipermeable membrane to prevent an immune response by the host immunosystem is a potential way of treating type I diabetes mellitus. In this study, poly(vinyl alcohol) (PVA) tubular membranes with added polyethylene glycol to create pores in the skin layer were prepared to improve their diffusion property. In a static incubation study, islets cultured in the PVA tubular membranes still demonstrated their function of secreting insulin after 30 days. When the tubular PVA bioartificial pancreas was perifused in a small chamber with RPMI-1640 medium containing glucose at concentrations of 5.6-16.6 mmol/L, insulin release began to increase without delay. Therefore, such a membrane is an alternative potential material for a bioartificial pancreas. In addition, a mathematical mass transfer model of insulin release was developed and compared with the perifusion data. It was shown that satisfactory kinetics could be achieved with a PVA membrane. However, the model showed that the insulin output of islets cultured in the PVA tubular membrane must be increased to improve the performance significantly. These findings suggest that a bioartificial pancreas using a PVA membrane is a promising material, but the technique for seeding islets in the chamber requires further modification.
Subjects
Bioartificial pancreas; Insulin release; Mass transfer model; Perifusion; PVA tubular membranes
SDGs
Other Subjects
Animal cell culture; Diffusion in solids; Glucose; Insulin; Mass transfer; Mathematical models; Polyethylene glycols; Polymeric membranes; Polyvinyl alcohols; Artificial pancreas; Artificial organs; pancrelipase; polyvinyl alcohol; animal tissue; article; artificial organ; diffusion; immune response; insulin dependent diabetes mellitus; insulin release; male; nonhuman; pancreas; pancreas islet; rat; Animals; Diffusion; Evaluation Studies; Insulin; Male; Membranes, Artificial; Models, Biological; Pancreas, Artificial; Perfusion; Polyvinyl Alcohol; Rats; Rats, Wistar
Type
journal article