Modulation of Pentylenetetrazol-Induced Bursting Activity by Electrogenic Na Pump in Achatina-Fulica Neurons
Resource
ASIA PACIFIC JOURNAL OF PHARMACOLOGY v.9 n.1 pp.37-42
Journal
ASIA PACIFIC JOURNAL OF PHARMACOLOGY
Journal Volume
v.9
Journal Issue
n.1
Pages
37-42
Date Issued
1994
Date
1994
Author(s)
V. L. ARVANOV
CHEN, RONG-CHI
CHEN, YI- HUNG
LIOU, HORNG-HUEI
CHANG, YANG-CHYUAN
V. A. ARVANOVA
TSAI, MING-CHENG
Abstract
Administration of pentylenetetrazol (PTZ) to neurons of Achatina fulica at room temperature induced characteristic bursting activity (BA) coincident with the seizure waves. The PTZ-induced BA disappeared after the temperature was lowered to 5 degrees C. Inhibition of electrogenic Na pump by both ouabain and/or K-free solution produced antagonism of PTZ induced BA. Incubation of the neurons in db-cAMP containing solution decreased the potency of the action of PTZ. The effects of PTZ on the membrane potential were not significantly affected by phorbol ester TPA, the activator of protein kinase C. Cd++ strongly inhibited the voltage activated Ca channels, but it had no effect on the PTZ- induced seizure waves. PTZ itself had no significant effect on the voltage-activated Ca current. These results indicate that electrogenic Na pump may be a strong modulator of the seizure waves induced by PTZ at the intact neurons. The modulation may be through Na pump induced changes of intracellular cAMP level.
Subjects
Pentylenetetrazol
Bursting activity
Electrogenic Na pump
cAMP
Ca current