Dual-Wavelength Photoacoustic Microscopy for Cell Tracking
Journal
the 172nd meeting of Acoustical Society of America
Date Issued
2016
Author(s)
Abstract
Understanding cell interactions can help to develop cell-based therapeutic strategies such as immunotherapy. The ability to track cells thus becomes an important task. 3D in vitro models have become a promising platform for such cell studies. However, conventional optical imaging methods are no longer adequate in 3D models due to limited penetration. To overcome this problem, we developed a dual-wavelength optical-resolution photoacoustic microscope (2L-OR-PAM) to study CD8 + cytotoxic T lymphocytes (CTLs) trafficking in a 3D tumor microenvironment. With the aid of molecular probes, the 2L-OR-PAM system can track both the CTLs and the tumor cells. An Nd:YLF laser was used as a pump laser at 523nm passing into a dye laser to generate near-infrared light at 813-nm. Two types of AuNPs were used to target the cancer cells and the CTLs. An x-y galvanometer scanner was employed to obtain a high scanning rate. The lateral and the axial resolutions were measured at 1.6 �gm and 5 �gm, respectively. We successfully showed that the 2L-OR-PAM can map the distribution of CTLs and 3D structures of Hepta1-6 tumor spheres. The 2L-OR-PAM can provide cellular and molecular information and it has the potential for preclinical screening requiring cell tracking.
Type
conference paper