Patterning Cell Cultures on Biodegradable Substrates by Inkjet Technology
Date Issued
2012
Date
2012
Author(s)
Ma, Yun-Ting
Abstract
In this research, we present an efficient and reliable approach to accurately control cell attachment positions on biodegradable substrates by inkjets printing technology. Sodium alginate cross-linked with calcium chloride is dried by casting /solvent evaporation method to prepare smooth square films. A drop-on-demand method is used to print various patterns of biodegradable material (PLA), such as circles, squares, and lines, which is a specific marker for 3T3-L1 cells. Cell growth profile, such as cell attachment, density and morphology, on various patterns are examined and studied by confocal fluorescence microscopy and optical microscopy. Smooth PLA pattern on calcium alginate film can be obtained when velocity of moving stage was set to 20
mm/s and the substrate temperature was kept at 30℃.
Previous studies showed that 3T3 cells prefer to attach to harder substrates. By this principle, to investigate effects of rigidity variation on cell attachment, multiple layers of PLA were printed on alginate to generate composite substrates with different PLA layer. The Young’s moduli of printed PLA layers on alginate are determined with AFM. Through AFM indentation, force–distance curves can be used to calculate Young’s moduli of composite films from Sneddon model. In the result, significantly more cells were attached to PLA with more printed PLA layers, or composite films with higher Young’s modulus. This rigidity-guided attachment approach can be used to create
cell culture on various patterns.
Subjects
Inkjets printing technology
Sodium alginate
poly lactic acid (PLA)
Biodegradable material
3T3-L1 cells
Type
thesis
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