Effect of Smoking on Exhaled Breath Volatile Organic Compounds
Date Issued
2007
Date
2007
Author(s)
Kao, Pei-Lin
DOI
zh-TW
Abstract
Breath analysis is a non-invasive biological monitoring method. Exhaled air pentane has been used as a marker of oxidative stress as well as urinary 8-hydroxy-2’-deoxyguanosine (8-OHdG). Because cigarette smoking can significantly increase the oxidative stress, this study used older smokers as the subjects and observed the effect of smoking on exhaled breath volatile organic compounds (VOCs) and 8-OHdG in urine.
A longitudinal study of the subjects （8 smokers, 8 non-smokers）was carried out with exhaled breath and urine sampling once every morning and recorded smokers’ daily cigarette consumption, time of the last smoking、alcohol intake and vitamin supplement. In addition, in smokers, exhaled breath was collected after cigarette cessation. Breath VOCs samples in Tedlar bags were preconcentrated with multi-bed sorbent sampling tubes and analyzed by thermal desorption-GC/MS. Urinary 8-OHdG was measured by ELISA. Mixed-effect models were used to investigate the breath VOCs difference between smokers and non-smokers by adjusting fixed effects and random effects.
We observed that exhaled breath VOCs increased immediately after smoking and were influenced by age and vitamin consumption. Using model adjusting these factors, 26 of 65 breath VOCs showed significant difference between smokers and non-smokers. Similar results were measured in smokers after cigarette cessation compared to non-smokers. Exhaled breath pentane in older smokers was still higher than non-smokers. In urinary 8-OHdG, smokers had 1.5-fold higher level than non-smokers. There was a correlation between exhaled breath pentane and urinary 8-OHdG in smokers.（r=0.55, p=0.06, n=12）.
In conclusion, cigarette smoking caused an immediate increase in exhaled breath VOCs which were also influenced by age and antioxidant supplement. Exhaled breath pentane as well as urinary 8-OHdG were used as a marker of oxidative stress and were increased in older smokers compared to non-smokers.
Subjects
抽菸
呼出氣體
揮發性有機物
氣相層析儀
混合型模式
Cigarette smoking
Exhaled breath
Volatile organic compounds(VOCs)
GC/MS
Mixed effect model
Type
thesis
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