A type II arabinogalactan from anoectochilus formosanus for G-CSF production in macrophages and leukopenia improvement in CT26-bearing mice treated with 5-fluorouracil
Journal
Evidence-based Complementary and Alternative Medicine
Journal Volume
2013
Date Issued
2013
Author(s)
Abstract
Anoectochilus formosanus is an herb well known in Asian countries. The polysaccharide isolated from A. formosanus consists of type II arabinogalactan (AGAF), with branched 3,6-Gal as the major moiety. In this study, AGAF was examined for the granulocyte colony-stimulating factor (G-CSF) production and related protein expression in RAW 264.7 murine macrophages. The signaling pathway of G-CSF production involves AGAF and mitogen-activated protein kinases (MAPKs) inhibitors and pattern-recognition receptor antibodies. AGAF was evaluated to ease the leukopenia in CT26-colon-cancer-bearing mice treated with 5-fluorouracil (5-FU). The results of this study showed that AGAF was a stimulant for Toll-like receptor 2 and Dectin-1 and that it induced G-CSF production, through p38 and ERK MAPK, and NF-B pathways. In vivo examination showed that the oral administration of AGAF mitigated the side effects of leukopenia caused by 5-FU in colon-cancer-bearing mice. In conclusion, the botanic type II AGAF in this study was a potent G-CSF inducer in vivo and in vitro. ? 2013 Li-Chan Yang et al.
SDGs
Other Subjects
arabinogalactan; dectin 1; fluorouracil; granulocyte colony stimulating factor; mitogen activated protein kinase; pattern recognition receptor; toll like receptor 2; animal cell; animal experiment; animal model; Anoectochilus formosanus; article; cell viability; colon cancer; comparative study; controlled study; cytokine production; enzyme phosphorylation; herb; leukocyte; leukocyte count; leukopenia; macrophage; male; mouse; nonhuman; priority journal; protein expression; spleen weight; Western blotting
Type
journal article
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