Cellulose-based diagnostic devices for diagnosing serotype-2 dengue fever in human serum
Journal
Advanced Healthcare Materials
Journal Volume
3
Journal Issue
2
Pages
187-196
Date Issued
2014
Author(s)
Wang, H.-K.
Tsai, C.-H.
Chen, K.-H.
Tang, C.-T.
Leou, J.-S.
Li, P.-C.
Tang, Y.-L.
Hsieh, H.-J.
Wu, H.-C.
Cheng, C.-M.
Abstract
Here, two types of cellulose-based in vitro diagnostic devices are demonstrated for the diagnosis of dengue virus infection in both buffer system and human serum: 1) paper-based ELISA for providing the semiquantitative information of the disease activity of serotype-2 dengue fever to healthcare persons (i.e., monitoring the disease activity with a specific serotype in single patients); 2) lateral flow immunoassays to screen for infection with serotype-2 dengue fever (i.e., rapid YES or NO diagnosis prepared for large populations, in terms of global public health). Paper-based ELISA (specific to serotype-2 dengue fever), which builds off of our previous studies and a revised previous ELISA procedure, owns multiple advantages: 1) high sensitivity (about 40 times higher than the current ELISA-based approaches, due to our therapeutic-based monoclonal antibody) and specificity (specific to dengue virus serotype-2 nonstructural protein-1 antigens); 2) tiny amount of sample and reagent used for single tests; 3) short operating duration (i.e., rapid diagnostic device); and, 4) inexpensiveness (appropriate for use in all developing and underdeveloped nations of the world). Due to the higher sensitivity and shorter operating duration of paper-based ELISA (compared with conventional ELISA, and lateral flow immunoassays also performed in this study), this study has not only been able to perform the diagnosis of dengue virus serotype-2 nonstructural protein-1 antigens in both buffer system and human serum but also to evaluate dengue virus serotype-2 envelope proteins in the buffer system, thus successfully achieving the first such use of these proteins as the target antigen for the development of diagnostic tools. These results provide a more comprehensive understanding for the genesis of dengue fever diagnostic tools (through antibody-antigen recognition). ? 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Subjects
Cellulose-based ELISA; Dengue fever; Lateral flow immunoassays; Rapid diagnosis
SDGs
Other Subjects
Antigen-antibody reactions; Antigens; Cellulose; Diagnostic products; Monoclonal antibodies; Viruses; Dengue fevers; Diagnostic device; Diagnostic tools; Envelope proteins; Global public health; In-vitro diagnostic; Lateral flow immunoassay; Nonstructural proteins; Diagnosis; buffer; dengue virus serotype 2 nonstructural protein 1 antigen; unclassified drug; virus antigen; virus protein; antigen antibody reaction; article; dengue; diagnostic test accuracy study; disease activity; ELISA kit; health care cost; hematology test kit; human; immunoassay; in vitro study; intermethod comparison; nonhuman; paper based ELISA kit; priority journal; protein structure; sensitivity and specificity; serotype; serotype 2 dengue fever; virus envelope; cellulose-based ELISA; dengue fever; lateral flow immunoassays; rapid diagnosis; Cellulose; Dengue; Enzyme-Linked Immunosorbent Assay; Humans; Immunoassay
Type
journal article