The effect of larval environments on the development and female reproduction in Culex quinquefasciatus & Culex pipiens molestus
Date Issued
2012
Date
2012
Author(s)
Huang, Kai-Kuo
Abstract
Autogenous Culex pipiens molestus is a good model for better understanding the relationship between oviposition and non-blood feeding signal. Vitellogenesis is the central step which involves the synthesis and secretion of yolk protein precursors (YPP) on a massive scale by the fat body in mosquito reproduction. In this thesis, the influence of larval rearing environment and Tebufenozide treatment on the growth of mosquito were compared by using two different mosquito species, autogenous (Cx. pipiens molestus) and anautogenous (Culex quinquefasciatus). Subsequently, RT-PCR analysis of Vg mRNA extracted from Cx. quinquefasciatus and Cx. pipiens molestus mosquito during a reproductive cycle will be presented. The results showed that tebufenozide treatment, abounding larval nourishment and low larval intensity have significantly impacted on the growth character and fecundity in both mosquitoes. However, rearing environment and Tebufenozide treatment have no effect on the anautogenous of mosquito. Moreover, vitellogenin genes (VgC1, VgC2) were only highly expressed after a bloodmeal. However, VgC1 sequence showed a 61 bp deletion during the period of high expression The effect of VgC1 sequence deletion on the reproduction of these two mosquitoes is currently unknow and deserves further investigation.
Subjects
Culex quinquefasciatus
Culex pipiens molestus
Autogenous
Anautogenous
vitellogenin
Type
thesis
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