Induction of apoptosis by [8]-shogaol via reactive oxygen species generation, glutathione depletion, and caspase activation in human leukemia cells
Journal
Journal of Agricultural and Food Chemistry
Journal Volume
58
Journal Issue
6
Pages
3847-3854
Date Issued
2010
Author(s)
Shieh, P.-C.
Chen, Y.-O.
Kuo, D.-H.
Chen, F.-A.
Tsai, M.-L.
Chang, I.-S.
Wu, H.
Sang, S.
Ho, C.-T.
Abstract
Ginger, the rhizome of Zingiber officinale , is a traditional medicine with a carminative effect and antinausea, anti-inflammatory, and anticarcinogenic properties. This study examined the growth inhibitory effects of [8]-shogaol, one of the pungent phenolic compounds in ginger, on human leukemia HL-60 cells. It demonstrated that [8]-shogaol was able to induce apoptosis in a time- and concentration-dependent manner. Treatment with [8]-shogaol caused a rapid loss of mitochondrial transmembrane potential, stimulation of reactive oxygen species (ROS) production, release of mitochondrial cytochrome c into cytosol, and subsequent induction of procaspase-9 and procaspase-3 processing. Taken together, these results suggest for the first time that ROS production and depletion of glutathione that contributed to [8]-shogaol-induced apoptosis in HL-60 cells.
SDGs
Type
journal article