Application of calcium on the control of lily gray mold and the mechanism study
Date Issued
2014
Date
2014
Author(s)
Chen, Yi-Ting
Abstract
Calcium is an essential element in plants and has important functions for plant defense. Calcium can bind calmodulin to modulate salicylic acid-mediated plant defense in Arabidopsis. The increase of calcium concentration can induce accumulation of hydrogen peroxide, further activate defense pathways. In practical application, foliar spray of calcium chloride (CaCl2) is known to suppress apple leaf scab. Lilies, a flower crop with economic importance in Taiwan, are often infected by Botrytis elliptica, a fungal pathogen of gray mold, that causes a servere loss in cut-flower production. Traditional control measure of lily gray mold is the use of chemical pesticides. To reduce the use of fungicides, foliar spray of micro-calcium carbonate (m-CaCO3) and CaCl2 instead of fungicides were applied to test their suppressive effect on lily gray mold and perform mechanism study of disease control in this study. In addition, lily ‘Stargazer’ transcriptome was established before analysis of the expression of defense-related genes after calcium treatment. The results of biocontrol assay indicated that m-CaCO3 and CaCl2 could suppress disease development of lily gray mold. Since spore germination and germ tube elongation of B. elliptica were not inhibited by m-CaCO3 and CaCl2, suppressing fungal attacke via activation of plant defense by these calcium compounds was presumed. The microscopical examination showed that callose deposition appeared earlier while inoculated with B. elliptica at one day after calcium treatment, indicating a priming effect of plant defense could be conducted by these calcium compounds. Reduction of hydrogen peroxide concentration supported suppressive effect on B. elliptica infection by these calcium compounds. However, increase of salicylic acid concentration and expressions of LsVSP2 and LsPR10 by micro-carbonate but not calcium chloride treatment indicated different defense pathways activated. In addition, the control efficacy of lily gray mold by micro-carbonate was further demonstrated in field assays. Thus, these calcium compounds are potential agents for the control of lily gray mold and can be developed as green materials for the reduction of fungicide use.
Subjects
百合灰黴病
微粒鈣
Botrytis elliptica
癒傷葡聚醣
過氧化氫
水楊酸
植物防禦相關基因
Type
thesis
File(s)![Thumbnail Image]()
Loading...
Name
ntu-103-R01633015-1.pdf
Size
23.54 KB
Format
Adobe PDF
Checksum
(MD5):54eb6af7b702229268bb635bdb4aea6b