Fabrication of multiresponsive bioactive nanocapsules through orthogonal self-assembly
Journal
Angewandte Chemie - International Edition
Journal Volume
53
Journal Issue
20
Pages
5137-5141
Date Issued
2014
Author(s)
Abstract
Multifunctional self-assembled systems present platforms for fundamental research and practical applications as they provide tunability of structure, functionality, and stimuli responsiveness. Pragmatic structures for biological applications have multiple design requirements, including control of size, stability, and environmental response. Here we present the fabrication of multifunctional nanoparticle-stabilized capsules (NPSCs) by using a set of orthogonal supramolecular interactions. In these capsules, fluorescent proteins are attached to quantum dots through polyhistidine coordination. These anionic assemblies interact laterally with cationic gold nanoparticles that are anchored to the fatty acid core through guanidinium-carboxylate interactions. The lipophilic core then provides a reservoir for hydrophobic endosome-disrupting agents, thereby generating a system featuring stimuli-responsive release of a payload into the cytosol with fluorescence monitoring. Multifunctional nanocapsules: Multifunctional nanoparticle-stabilized capsules (NPSCs) have been fabricated by using a set of orthogonal supramolecular interactions. The resultant supramolecular nanocapsules feature dual-delivery capability of proteins and hydrophobic endosome-disrupting agents to allow stimuli-responsive protein release into the cytosol with fluorescence monitoring. ? 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Subjects
nanocapsules
nanoparticles
noncovalent interactions
self-assembly
stimuli-responsive release
SDGs
Type
journal article