Real-time assay of immobilized tannase with a stopped-flow conductometric device
Resource
Bioelectrochemistry 69, 113–116
Journal
Bioelectrochemistry 69, 113–116
Pages
-
Date Issued
2006-02-07
Date
2006-02-07
Author(s)
DOI
246246/2006111501264990
Abstract
A stopped-flow manifold was developed to assay and characterize immobilized tannase (EC 3.1.1.20). The immobilized enzyme reactor was
inserted within the tube-type electrode pair (cell constant=103.2 cm−1) for a real-time conductometric measurement. Tris buffer (2 mM, pH=7.0)
was used as the carrier for sensitivity improvement. The activities and kinetic parameters (Km values) for propyl gallate, methyl gallate and tannic
acid were investigated.
Subjects
Conductometric
Enzyme kinetics
Tannic acid
Stopped-flow
Publisher
Taipei:National Taiwan University Coll Management
Type
journal article
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