Frequency Scan of a Quadrupole Mass Analyzer in the Third Stability Region for Protein Analysis and Development of Absolute Charge State Assignment Program
Date Issued
2005
Date
2005
Author(s)
Lin, Chung-Wei
DOI
en-US
Abstract
Part A: A linear quadrupole mass spectrometer operating in the third stability region [(a, q) ≈ (3, 3)] has been developed and utilized to obtain the mass spectra of multiply charged protein ions generated by electrospray ionization. The spectra were acquired by scanning the frequency of the quadrupole with a home-built wide-band power amplifier from 400 to 100 kHz at constant voltage amplitudes in the range of 100 V. A resolution approaching R1/2 = 200 was achieved for doubly charged gramicidin S ions at m/z 570.5. Besides the experiments, a computer simulation was utilized to discuss the results of experiments. Further improvement of the mass resolution is expected when a higher voltage power amplifier is used.
Part B: A new technique for determining the masses of nanoparticles in a quadrupole ion trap has been developed in our group. When a particle is introduced into the ion trap and trapped, mass-to-charge ratio of the particle is determined by varying the voltage and RF frequency. Computer programs were utilized to speed the data analysis. Two kinds of programs were compared to testify the validity of data analysis.
Subjects
四極柱質譜儀
電灑離子法
quadrupole
ESI
Type
thesis
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