Sub-micron Electrically Programmable Protein Patterning Technology
Date Issued
2008
Date
2008
Author(s)
Lu, Cheng-Wei
Abstract
Bio-sensor is one of the fundamental technologies for Proteomics. With the technology of the Micro- and Nano- Electromechanical Systems, it can be easily to manufacture the miniature portable device, that include the surface plasmon resonance detector, piezoelectric quartz crystal, ion-selective field effect transistor and eletrochemical bio-sensor. Protein patterning is a method which makes those devices more selective and sensitive. Protein patterning technology is to make proteins or bio-molecules be defined on some specific areas. Using some kinds of the patterning technologies to let the bio-molecules immobilized on a substrate, and then make the assays or reagents to be interact with the patterned bio-molecules, with this method it can be fleetly and enormously analysed the interact between the unknown lysate, bio-molecules and chemicals. The patterned object could be the proteins, DANs and all kinds of bio-molecules. The application of the protein patterning can be used widely, including the genome, cancer research, drug discovery, clinical diagnostics, cell studies, tissue engineering and bio-molecule identification.owadays, the protein patterning technologies are hard to achieve the high resolution and programmable patterns, moreover those methods might cause the patterned proteins physical or chemical damages. So this study is using the micro- and nano-electromechanical systems to manufacture the submicron electrically programmable protein patterning device, and utilizing the fluorescence labeling process and fluorescent microscope to analyse the patterned result. This method can not only make high resolution programmable patterns effectively without denaturing those proteins, but also sustain their activities to make sure the feasible of applications.
Subjects
Protein Patterning
programmable
submicron
SDGs
Type
thesis
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