The effects of dietary lysine deficiency on muscle protein turnover in postweanling pigs
Resource
Animal Science, 18(9), 1326-1335
Journal
Asian-Australasian Journal of Animal Sciences
Journal Volume
18
Journal Issue
9
Pages
1326-1335
Date Issued
2005
Author(s)
Chang, Y.-M.
Abstract
The main purpose of this study is to investigate the effects of dietary lysine deficiency on protein turnover of porcine muscles. There were 18 LYD three-breed-crossing postweanling barrows from six litters cannulated with gastric tubes through the esophagus at approximate 10 kg of body weight and allocated into three treatment groups. When their body weights reached over 12 kg, one group was sacrificed for determining the initial protein masses of m. masseter, m. longissimus dorsi, m. adductor and m. biceps femoris from the right body side. The others received a diet containing 100% or 61.4% (calculated values) of the lysine requirement (NRC, 1998) multiplied by 1.103 for a period of 17 days. Daily feed provision was computed for each pig according to body weight at the same day. All pigs were infused a flooding dose of [ 2 H 5 ]-phenylalanine to determine the fractional protein synthesis rates (FSR) of the aforementioned muscles in the end. Their four muscles from the right body side were also dissected for measuring the fractional rates of protein accretion (FAR). As for protein degradation, fractional rates (FDR) were calculated by differences between synthesis and accretion. Results showed that the lysine deficiency resulted in, significantly (p0.05), nevertheless, in the amino-acid composition of muscles between two groups. The lysine deficiency reduced significantly (p0.05). In conclusion, the FAR of muscle protein was changed by the current lysine deficiency through the alterations in the FSR and/or FDR. (
Type
journal article